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DOI | 10.1126/science.aau1783 |
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells | |
Bintu, Bogdan1,2; Mateo, Leslie J.3; Su, Jun-Han1,2; Sinnott-Armstrong, Nicholas A.4; Parker, Mirae5,6; Kinrot, Seon1,2; Yamaya, Kei3; Boettiger, Alistair N.3; Zhuang, Xiaowei1,2 | |
2018-10-26 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6413页码:419-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The spatial organization of chromatin is pivotal for regulating genome functions. We report an imaging method for tracing chromatin organization with kilobase-and nanometer-scale resolution, unveiling chromatin conformation across topologically associating domains (TADs) in thousands of individual cells. Our imaging data revealed TAD-like structures with globular conformation and sharp domain boundaries in single cells. The boundaries varied from cell to cell, occurring with nonzero probabilities at all genomic positions but preferentially at CCCTC-binding factor (CTCF)-and cohesin-binding sites. Notably, cohesin depletion, which abolished TADs at the population-average level, did not diminish TAD-like structures in single cells but eliminated preferential domain boundary positions. Moreover, we observed widespread, cooperative, multiway chromatin interactions, which remained after cohesin depletion. These results provide critical insight into the mechanisms underlying chromatin domain and hub formation. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000450441900038 |
WOS关键词 | OPTICAL RECONSTRUCTION MICROSCOPY ; MAMMALIAN GENOMES ; 3D GENOME ; HI-C ; SPATIAL-ORGANIZATION ; DROSOPHILA GENOME ; COHESIN ; TRANSCRIPTION ; CHROMOSOME ; PRINCIPLES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199924 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Univ, Howard Hughes Med Inst, Dept Chem & Chem Biol, Cambridge, MA 02138 USA; 2.Harvard Univ, Dept Phys, Cambridge, MA 02138 USA; 3.Stanford Univ, Dept Dev Biol, Stanford, CA 94305 USA; 4.Stanford Univ, Dept Genet, Stanford, CA 94305 USA; 5.Stanford Univ, Dept Phys, Stanford, CA 94305 USA; 6.MIT, Dept Computat Syst Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA |
推荐引用方式 GB/T 7714 | Bintu, Bogdan,Mateo, Leslie J.,Su, Jun-Han,et al. Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells[J]. SCIENCE,2018,362(6413):419-+. |
APA | Bintu, Bogdan.,Mateo, Leslie J..,Su, Jun-Han.,Sinnott-Armstrong, Nicholas A..,Parker, Mirae.,...&Zhuang, Xiaowei.(2018).Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.SCIENCE,362(6413),419-+. |
MLA | Bintu, Bogdan,et al."Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells".SCIENCE 362.6413(2018):419-+. |
条目包含的文件 | 条目无相关文件。 |
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