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DOI | 10.1126/science.aat1674 |
Generation of human oogonia from induced pluripotent stem cells in vitro | |
Yamashiro, Chika1,2; Sasaki, Kotaro1,2; Yabuta, Yukihiro1,2; Kojima, Yoji1,2,3,4; Nakamura, Tomonori1,2; Okamoto, Ikuhiro1,2; Yokobayashi, Shihori1,2,4; Murase, Yusuke1,2; Ishikura, Yukiko1,2; Shirane, Kenjiro5,6,7; Sasaki, Hiroyuki5,6,7; Yamamoto, Takuya3,4,8; Saitou, Mitinori1,2,3,4 | |
2018-10-19 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6412页码:356-359 |
文章类型 | Article |
语种 | 英语 |
国家 | Japan |
英文摘要 | Human in vitro gametogenesis may transform reproductive medicine. Human pluripotent stem cells (hPSCs) have been induced into primordial germ cell-like cells (hPGCLCs); however, further differentiation to a mature germ cell has not been achieved. Here, we show that hPGCLCs differentiate progressively into oogonia-like cells during a long-term in vitro culture (approximately 4 months) in xenogeneic reconstituted ovaries with mouse embryonic ovarian somatic cells. The hPGCLC-derived oogonia display hallmarks of epigenetic reprogramming-genome-wide DNA demethylation, imprint erasure, and extinguishment of aberrant DNA methylation in hPSCs-and acquire an immediate precursory state for meiotic recombination. Furthermore, the inactive X chromosome shows a progressive demethylation and reactivation, albeit partially. These findings establish the germline competence of hPSCs and provide a critical step toward human in vitro gametogenesis. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000447680100052 |
WOS关键词 | GERM-CELLS ; FATE ; MICE ; RECONSTITUTION ; SPECIFICATION ; INDUCTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199876 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Kyoto Univ, Grad Sch Med, Dept Anat & Cell Biol, Sakyo Ku, Yoshida Konoe Cho, Kyoto 6068501, Japan; 2.Japan Sci & Technol Agcy JST, Exploratory Res Adv Technol ERATO, Sakyo Ku, Yoshida Konoe Cho, Kyoto 6068501, Japan; 3.Kyoto Univ, Inst Integrated Cell Mat Sci, Sakyo Ku, Yoshida Ushinomiya Cho, Kyoto 6068501, Japan; 4.Kyoto Univ, Ctr iPS Cell Res & Applicat, Sakyo Ku, 53 Kawahara Cho, Shogoin, Kyoto 6068507, Japan; 5.Kyushu Univ, Div Epigen, Med Inst Bioregulat, Higashi Ku, Maidashi 3-1-1, Fukuoka, Fukuoka 8128582, Japan; 6.Kyushu Univ, Epigen Network Res Ctr, Higashi Ku, Maidashi 3-1-1, Fukuoka, Fukuoka 8128582, Japan; 7.Kyushu Univ, Grad Sch Med Sci, Higashi Ku, Maidashi 3-1-1, Fukuoka, Fukuoka 8128582, Japan; 8.Agcy Med Res & Dev, CREST, AMED, Chiyoda Ku, 1-7-1 Otemachi, Tokyo 1000004, Japan |
推荐引用方式 GB/T 7714 | Yamashiro, Chika,Sasaki, Kotaro,Yabuta, Yukihiro,et al. Generation of human oogonia from induced pluripotent stem cells in vitro[J]. SCIENCE,2018,362(6412):356-359. |
APA | Yamashiro, Chika.,Sasaki, Kotaro.,Yabuta, Yukihiro.,Kojima, Yoji.,Nakamura, Tomonori.,...&Saitou, Mitinori.(2018).Generation of human oogonia from induced pluripotent stem cells in vitro.SCIENCE,362(6412),356-359. |
MLA | Yamashiro, Chika,et al."Generation of human oogonia from induced pluripotent stem cells in vitro".SCIENCE 362.6412(2018):356-359. |
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