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DOI | 10.1126/science.aau5138 |
Systematic discovery of natural CRISPR-Cas12a inhibitors | |
Watters, Kyle E.1; Fellmann, Christof1,2; Bai, Hua B.1; Ren, Shawn M.1; Doudna, Jennifer A.1,2,3,4,5,6,7 | |
2018-10-12 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6411页码:236-239 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Cas12a (Cpf1) is a CRISPR-associated nuclease with broad utility for synthetic genome engineering, agricultural genomics, and biomedical applications. Although bacteria harboring CRISPR-Cas9 or CRISPR-Cas3 adaptive immune systems sometimes acquire mobile genetic elements encoding anti-CRISPR proteins that inhibit Cas9, Cas3, or the DNA-binding Cascade complex, no such inhibitors have been found for CRISPR-Cas12a. Here we use a comprehensive bioinformatic and experimental screening approach to identify three different inhibitors that block or diminish CRISPR-Cas12a-mediated genome editing in human cells. We also find a widespread connection between CRISPR self-targeting and inhibitor prevalence in prokaryotic genomes, suggesting a straightforward path to the discovery of many more anti-CRISPRs from the microbial world. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000447337200018 |
WOS关键词 | CRISPR-CAS ; ANTI-CRISPR ; RNA ; ACRIIA4 |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199810 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA; 2.Gladstone Inst, San Francisco, CA 94158 USA; 3.Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA; 4.Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94720 USA; 5.Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA; 6.Univ Calif Berkeley, Li Ka Shing Biomed & Hlth Sci Ctr, Berkeley, CA 94720 USA; 7.Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA |
推荐引用方式 GB/T 7714 | Watters, Kyle E.,Fellmann, Christof,Bai, Hua B.,et al. Systematic discovery of natural CRISPR-Cas12a inhibitors[J]. SCIENCE,2018,362(6411):236-239. |
APA | Watters, Kyle E.,Fellmann, Christof,Bai, Hua B.,Ren, Shawn M.,&Doudna, Jennifer A..(2018).Systematic discovery of natural CRISPR-Cas12a inhibitors.SCIENCE,362(6411),236-239. |
MLA | Watters, Kyle E.,et al."Systematic discovery of natural CRISPR-Cas12a inhibitors".SCIENCE 362.6411(2018):236-239. |
条目包含的文件 | 条目无相关文件。 |
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