Discovery of widespread type I and type V CRISPR-Cas inhibitors

doi:10.1126/science.aau5174

GSTDTAP > 地球科学

DOI	10.1126/science.aau5174
	Discovery of widespread type I and type V CRISPR-Cas inhibitors
	Marino, Nicole D.1; Zhang, Jenny Y.1; Borges, Adair L.1; Sousa, Alexander A.2,3,4; Leon, Lina M.1; Rauch, Benjamin J.1,7; Walton, Russell T.2,3,4; Berry, Joel D.1,8; Joung, J. Keith 2,3,4,5; Kleinstiver, Benjamin P.2,3,4,5; Bondy-Denomy, Joseph 1,6
	2018-10-12
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2018
卷号	362 期号:6411 页码:240-242
文章类型	Article
语种	英语
国家	USA
英文摘要	Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit the immune function of CRISPR-Cas. To date, Acr proteins have been discovered for type I (subtypes I-D, I-E, and I-F) and type II (II-A and II-C) but not other CRISPR systems. Here, we report the discovery of 12 acr genes, including inhibitors of type V-A and I-C CRISPR systems. AcrVA1 inhibits a broad spectrum of Cas12a (Cpf1) orthologs-including MbCas12a, Mb3Cas12a, AsCas12a, and LbCas12a-when assayed in human cells. The acr genes reported here provide useful biotechnological tools and mark the discovery of acr loci in many bacteria and phages.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000447337200058
WOS关键词	INFECTIOUS BOVINE KERATOCONJUNCTIVITIS ; CPF1 ; MECHANISMS ; PROTEINS ; SYSTEM ; RNA
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/199809
专题	地球科学资源环境科学气候变化
作者单位	1.Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA; 2.Massachusetts Gen Hosp, Mol Pathol Unit, Charlestown, MA 02129 USA; 3.Massachusetts Gen Hosp, Ctr Canc Res, Charlestown, MA 02129 USA; 4.Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Charlestown, MA 02129 USA; 5.Harvard Med Sch, Dept Pathol, Boston, MA 02115 USA; 6.Univ Calif San Francisco, Quantitat Biosci Inst, San Francisco, CA 94143 USA; 7.Zymergen, Emeryville, CA 94608 USA; 8.Caribou Biosci, Berkeley, CA 94710 USA
推荐引用方式 GB/T 7714	Marino, Nicole D.,Zhang, Jenny Y.,Borges, Adair L.,et al. Discovery of widespread type I and type V CRISPR-Cas inhibitors[J]. SCIENCE,2018,362(6411):240-242.
APA	Marino, Nicole D..,Zhang, Jenny Y..,Borges, Adair L..,Sousa, Alexander A..,Leon, Lina M..,...&Bondy-Denomy, Joseph.(2018).Discovery of widespread type I and type V CRISPR-Cas inhibitors.SCIENCE,362(6411),240-242.
MLA	Marino, Nicole D.,et al."Discovery of widespread type I and type V CRISPR-Cas inhibitors".SCIENCE 362.6411(2018):240-242.