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DOI | 10.1126/science.aau5174 |
Discovery of widespread type I and type V CRISPR-Cas inhibitors | |
Marino, Nicole D.1; Zhang, Jenny Y.1; Borges, Adair L.1; Sousa, Alexander A.2,3,4; Leon, Lina M.1; Rauch, Benjamin J.1,7; Walton, Russell T.2,3,4; Berry, Joel D.1,8; Joung, J. Keith2,3,4,5; Kleinstiver, Benjamin P.2,3,4,5; Bondy-Denomy, Joseph1,6 | |
2018-10-12 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 362期号:6411页码:240-242 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Bacterial CRISPR-Cas systems protect their host from bacteriophages and other mobile genetic elements. Mobile elements, in turn, encode various anti-CRISPR (Acr) proteins to inhibit the immune function of CRISPR-Cas. To date, Acr proteins have been discovered for type I (subtypes I-D, I-E, and I-F) and type II (II-A and II-C) but not other CRISPR systems. Here, we report the discovery of 12 acr genes, including inhibitors of type V-A and I-C CRISPR systems. AcrVA1 inhibits a broad spectrum of Cas12a (Cpf1) orthologs-including MbCas12a, Mb3Cas12a, AsCas12a, and LbCas12a-when assayed in human cells. The acr genes reported here provide useful biotechnological tools and mark the discovery of acr loci in many bacteria and phages. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000447337200058 |
WOS关键词 | INFECTIOUS BOVINE KERATOCONJUNCTIVITIS ; CPF1 ; MECHANISMS ; PROTEINS ; SYSTEM ; RNA |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199809 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94143 USA; 2.Massachusetts Gen Hosp, Mol Pathol Unit, Charlestown, MA 02129 USA; 3.Massachusetts Gen Hosp, Ctr Canc Res, Charlestown, MA 02129 USA; 4.Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Charlestown, MA 02129 USA; 5.Harvard Med Sch, Dept Pathol, Boston, MA 02115 USA; 6.Univ Calif San Francisco, Quantitat Biosci Inst, San Francisco, CA 94143 USA; 7.Zymergen, Emeryville, CA 94608 USA; 8.Caribou Biosci, Berkeley, CA 94710 USA |
推荐引用方式 GB/T 7714 | Marino, Nicole D.,Zhang, Jenny Y.,Borges, Adair L.,et al. Discovery of widespread type I and type V CRISPR-Cas inhibitors[J]. SCIENCE,2018,362(6411):240-242. |
APA | Marino, Nicole D..,Zhang, Jenny Y..,Borges, Adair L..,Sousa, Alexander A..,Leon, Lina M..,...&Bondy-Denomy, Joseph.(2018).Discovery of widespread type I and type V CRISPR-Cas inhibitors.SCIENCE,362(6411),240-242. |
MLA | Marino, Nicole D.,et al."Discovery of widespread type I and type V CRISPR-Cas inhibitors".SCIENCE 362.6411(2018):240-242. |
条目包含的文件 | 条目无相关文件。 |
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