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DOI | 10.1126/science.aap8987 |
Cellular checkpoint control using programmable sequential logic | |
Andrews, Lauren B.1,2,3; Nielsen, Alec A. K.2; Voigt, Christopher A.1,2 | |
2018-09-21 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6408页码:1217-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Biological processes that require orderly progression, such as growth and differentiation, proceed via regulatory checkpoints where the cell waits for signals before continuing to the next state. Implementing such control would allow genetic engineers to divide complex tasks into stages. We present genetic circuits that encode sequential logic to instruct Escherichia coli to proceed through a linear or cyclical sequence of states. These are built with 11 set-reset latches, designed with repressor-based NOR gates, which can connect to each other and sensors. The performance of circuits with up to three latches and four sensors, including a gated D latch, closely match predictions made by using nonlinear dynamics. Checkpoint control is demonstrated by switching cells between multiple circuit states in response to external signals over days. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000445152500031 |
WOS关键词 | SYNTHETIC BIOLOGY ; POSITIVE FEEDBACK ; REGULATORY NETWORKS ; ESCHERICHIA-COLI ; GENETIC MEMORY ; CIRCUITS ; BISTABILITY ; SWITCH ; CELLS ; SYSTEMS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199634 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Broad Inst MIT & Harvard, Cambridge, MA 02142 USA; 2.MIT, Synthet Biol Ctr, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 3.Univ Massachusetts, Dept Chem Engn, Amherst, MA 01003 USA |
推荐引用方式 GB/T 7714 | Andrews, Lauren B.,Nielsen, Alec A. K.,Voigt, Christopher A.. Cellular checkpoint control using programmable sequential logic[J]. SCIENCE,2018,361(6408):1217-+. |
APA | Andrews, Lauren B.,Nielsen, Alec A. K.,&Voigt, Christopher A..(2018).Cellular checkpoint control using programmable sequential logic.SCIENCE,361(6408),1217-+. |
MLA | Andrews, Lauren B.,et al."Cellular checkpoint control using programmable sequential logic".SCIENCE 361.6408(2018):1217-+. |
条目包含的文件 | 条目无相关文件。 |
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