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DOI | 10.1126/science.aat2477 |
Ribonucleotide incorporation enables repair of chromosome breaks by nonhomologous end joining | |
Pryor, John M.1,2; Conlin, Michael P.1; Carvajal-Garcia, Juan1; Luedeman, Megan E.1; Luthman, Adam J.1; Small, George W.1; Ramsden, Dale A.1 | |
2018-09-14 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6407页码:1126-1129 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | The nonhomologous end-joining (NHEJ) pathway preserves genome stability by ligating the ends of broken chromosomes together. It employs end-processing enzymes, including polymerases, to prepare ends for ligation. We show that two such polymerases incorporate primarily ribonucleotides during NHEJ-an exception to the central dogma of molecular biology-both during repair of chromosome breaks made by Cas9 and during V(D)J recombination. Moreover, additions of ribonucleotides but not deoxynucleotides effectively promote ligation. Repair kinetics suggest that ribonucleotide-dependent first-strand ligation is followed by complementary strand repair with deoxynucleotides, then by replacement of ribonucleotides embedded in the first strand with deoxynucleotides. Our results indicate that as much as 65% of cellular NHEJ products have transiently embedded ribonucleotides, which promote flexibility in repair at the cost of more fragile intermediates. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000444513300044 |
WOS关键词 | DNA-POLYMERASE-MU ; V(D)J RECOMBINATION ; DEFICIENT ; CELLS ; TDT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199584 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ N Carolina, Lineberger Comprehens Canc Ctr, Dept Biochem & Biophys & Curriculum Genet & Mol B, Chapel Hill, NC 27599 USA; 2.New England Biolabs Inc, Ipswich, MA 01938 USA |
推荐引用方式 GB/T 7714 | Pryor, John M.,Conlin, Michael P.,Carvajal-Garcia, Juan,et al. Ribonucleotide incorporation enables repair of chromosome breaks by nonhomologous end joining[J]. SCIENCE,2018,361(6407):1126-1129. |
APA | Pryor, John M..,Conlin, Michael P..,Carvajal-Garcia, Juan.,Luedeman, Megan E..,Luthman, Adam J..,...&Ramsden, Dale A..(2018).Ribonucleotide incorporation enables repair of chromosome breaks by nonhomologous end joining.SCIENCE,361(6407),1126-1129. |
MLA | Pryor, John M.,et al."Ribonucleotide incorporation enables repair of chromosome breaks by nonhomologous end joining".SCIENCE 361.6407(2018):1126-1129. |
条目包含的文件 | 条目无相关文件。 |
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