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DOI | 10.1126/science.aat8474 |
A designed heme-[4Fe-4S] metalloenzyme catalyzes sulfite reduction like the native enzyme | |
Mirts, Evan N.1; Petrik, Igor D.2; Hosseinzadeh, Parisa3; Nilges, Mark J.4; Lu, Yi1,2,3,5,6,7 | |
2018-09-14 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6407页码:1098-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Multielectron redox reactions often require multicofactor metalloenzymes to facilitate coupled electron and proton movement, but it is challenging to design artificial enzymes to catalyze these important reactions, owing to their structural and functional complexity. We report a designed heteronuclear heme-[4Fe-4S] cofactor in cytochrome c peroxidase as a structural and functional model of the enzyme sulfite reductase. The initial model exhibits spectroscopic and ligand-binding properties of the native enzyme, and sulfite reduction activity was improved-through rational tuning of the secondary sphere interactions around the [4Fe-4S] and the substrate-binding sites-to be close to that of the native enzyme. By offering insight into the requirements for a demanding six-electron, seven-proton reaction that has so far eluded synthetic catalysts, this study provides strategies for designing highly functional multicofactor artificial enzymes. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000444513300036 |
WOS关键词 | ELECTRON-PARAMAGNETIC-RESONANCE ; FE4S4 PROSTHETIC GROUPS ; ACTIVE-SITE ; HEMOPROTEIN ; COMPLEXES ; SIROHEME ; CYANIDE ; CYTOCHROME-P450 ; PERCHLORATE ; POTENTIALS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199577 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Illinois, Ctr Biophys & Quantitat Biol, Urbana, IL 61801 USA; 2.Univ Illinois, Dept Chem, Urbana, IL 61801 USA; 3.Univ Illinois, Dept Biochem, Urbana, IL 61801 USA; 4.Univ Illinois, Sch Chem Sci, Electron Paramagnet Resonance Lab, Urbana, IL 61801 USA; 5.Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA; 6.Univ Illinois, DOE Ctr Adv Bioenergy & Bioprod Innovat, Urbana, IL 61801 USA; 7.Pacific Northwest Natl Lab, Richland, WA 99352 USA |
推荐引用方式 GB/T 7714 | Mirts, Evan N.,Petrik, Igor D.,Hosseinzadeh, Parisa,et al. A designed heme-[4Fe-4S] metalloenzyme catalyzes sulfite reduction like the native enzyme[J]. SCIENCE,2018,361(6407):1098-+. |
APA | Mirts, Evan N.,Petrik, Igor D.,Hosseinzadeh, Parisa,Nilges, Mark J.,&Lu, Yi.(2018).A designed heme-[4Fe-4S] metalloenzyme catalyzes sulfite reduction like the native enzyme.SCIENCE,361(6407),1098-+. |
MLA | Mirts, Evan N.,et al."A designed heme-[4Fe-4S] metalloenzyme catalyzes sulfite reduction like the native enzyme".SCIENCE 361.6407(2018):1098-+. |
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