GSTDTAP  > 地球科学
DOI10.1126/science.aat9804
Developmental barcoding of whole mouse via homing CRISPR
Kalhor, Reza1,2; Kalhor, Kian3; Mejia, Leo1; Leeper, Kathleen2; Graveline, Amanda2; Mali, Prashant4; Church, George M.1,2
2018-08-31
发表期刊SCIENCE
ISSN0036-8075
EISSN1095-9203
出版年2018
卷号361期号:6405页码:893-+
文章类型Article
语种英语
国家USA; Iran
英文摘要

In vivo barcoding using nuclease-induced mutations is a powerful approach for recording biological information, including developmental lineages; however, its application in mammalian systems has been limited. We present in vivo barcoding in the mouse with multiple homing guide RNAs that each generate hundreds of mutant alleles and combine to produce an exponential diversity of barcodes. Activation upon conception and continued mutagenesis through gestation resulted in developmentally barcoded mice wherein information is recorded in lineage-specific mutations. We used these recordings for reliable post hoc reconstruction of the earliest lineages and investigation of axis development in the brain. Our results provide an enabling and versatile platform for in vivo barcoding and lineage tracing in a mammalian model system.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000443547000032
WOS关键词DNA CLEAVAGE ; CELL LINEAGE ; CLONAL ANALYSIS ; SINGLE CELLS ; GENOMIC DNA ; IN-SITU ; EMBRYO ; SYSTEM ; RNA ; MICE
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
被引频次:237[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/199474
专题地球科学
资源环境科学
气候变化
作者单位1.Harvard Med Sch, Dept Genet, Boston, MA 02115 USA;
2.Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA;
3.Sharif Univ Technol, Dept Elect Engn, Tehran, Iran;
4.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA
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GB/T 7714
Kalhor, Reza,Kalhor, Kian,Mejia, Leo,et al. Developmental barcoding of whole mouse via homing CRISPR[J]. SCIENCE,2018,361(6405):893-+.
APA Kalhor, Reza.,Kalhor, Kian.,Mejia, Leo.,Leeper, Kathleen.,Graveline, Amanda.,...&Church, George M..(2018).Developmental barcoding of whole mouse via homing CRISPR.SCIENCE,361(6405),893-+.
MLA Kalhor, Reza,et al."Developmental barcoding of whole mouse via homing CRISPR".SCIENCE 361.6405(2018):893-+.
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