Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aat9804 |
Developmental barcoding of whole mouse via homing CRISPR | |
Kalhor, Reza1,2; Kalhor, Kian3; Mejia, Leo1; Leeper, Kathleen2; Graveline, Amanda2; Mali, Prashant4; Church, George M.1,2 | |
2018-08-31 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6405页码:893-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Iran |
英文摘要 | In vivo barcoding using nuclease-induced mutations is a powerful approach for recording biological information, including developmental lineages; however, its application in mammalian systems has been limited. We present in vivo barcoding in the mouse with multiple homing guide RNAs that each generate hundreds of mutant alleles and combine to produce an exponential diversity of barcodes. Activation upon conception and continued mutagenesis through gestation resulted in developmentally barcoded mice wherein information is recorded in lineage-specific mutations. We used these recordings for reliable post hoc reconstruction of the earliest lineages and investigation of axis development in the brain. Our results provide an enabling and versatile platform for in vivo barcoding and lineage tracing in a mammalian model system. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000443547000032 |
WOS关键词 | DNA CLEAVAGE ; CELL LINEAGE ; CLONAL ANALYSIS ; SINGLE CELLS ; GENOMIC DNA ; IN-SITU ; EMBRYO ; SYSTEM ; RNA ; MICE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199474 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Med Sch, Dept Genet, Boston, MA 02115 USA; 2.Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA; 3.Sharif Univ Technol, Dept Elect Engn, Tehran, Iran; 4.Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA |
推荐引用方式 GB/T 7714 | Kalhor, Reza,Kalhor, Kian,Mejia, Leo,et al. Developmental barcoding of whole mouse via homing CRISPR[J]. SCIENCE,2018,361(6405):893-+. |
APA | Kalhor, Reza.,Kalhor, Kian.,Mejia, Leo.,Leeper, Kathleen.,Graveline, Amanda.,...&Church, George M..(2018).Developmental barcoding of whole mouse via homing CRISPR.SCIENCE,361(6405),893-+. |
MLA | Kalhor, Reza,et al."Developmental barcoding of whole mouse via homing CRISPR".SCIENCE 361.6405(2018):893-+. |
条目包含的文件 | 条目无相关文件。 |
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