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DOI | 10.1126/science.aau8230 |
Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water" | |
Fuertes, Gustavo1,2,3; Banterle, Niccolo1; Ruff, Kiersten M.4,5; Chowdhury, Aritra1; Pappu, Rohit V.4,5; Svergun, Dmitri I.2; Lemke, Edward A.1,6,7,8,9 | |
2018-08-31 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6405 |
文章类型 | Editorial Material |
语种 | 英语 |
国家 | Germany; Czech Republic; USA |
英文摘要 | Editors at Science requested our input on the above discussion (comment by Best et al. and response by Riback et al.) because both sets of authors use our data from Fuertes et al. (2017) to support their arguments. The topic of discussion pertains to the discrepant inferences drawn from SAXS versus FRET measurements regarding the dimensions of intrinsically disordered proteins (IDPs) in aqueous solvents. Using SAXS measurements on labeled and unlabeled proteins, we ruled out the labels used for FRET measurements as the cause of discrepant inferences between the two methods. Instead, we propose that FRET and SAXS provide complementary readouts because of a decoupling of size and shape fluctuations that is intrinsic to finite-sized, heteropolymeric IDPs. Accounting for this decoupling resolves the discrepant inferences between the two methods, thus making a case for the utility of both methods. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E ; SSCI |
WOS记录号 | WOS:000443547000004 |
WOS关键词 | FRET MEASUREMENTS ; DIMENSIONS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199463 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.European Mol Biol Lab, D-69117 Heidelberg, Germany; 2.European Mol Biol Lab, D-22607 Hamburg, Germany; 3.Inst Biotechnol CAS, Vestec 25250, Czech Republic; 4.Washington Univ, Dept Biomed Engn, St Louis, MO 63130 USA; 5.Washington Univ, Ctr Biol Syst Engn, St Louis, MO 63130 USA; 6.Johannes Gutenberg Univ Mainz, Bioctr, Dept Biol, D-55128 Mainz, Germany; 7.Johannes Gutenberg Univ Mainz, Bioctr, Dept Chem, D-55128 Mainz, Germany; 8.Johannes Gutenberg Univ Mainz, Bioctr, Dept Pharm & Geosci, D-55128 Mainz, Germany; 9.Inst Mol Biol, D-55128 Mainz, Germany |
推荐引用方式 GB/T 7714 | Fuertes, Gustavo,Banterle, Niccolo,Ruff, Kiersten M.,et al. Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water"[J]. SCIENCE,2018,361(6405). |
APA | Fuertes, Gustavo.,Banterle, Niccolo.,Ruff, Kiersten M..,Chowdhury, Aritra.,Pappu, Rohit V..,...&Lemke, Edward A..(2018).Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water".SCIENCE,361(6405). |
MLA | Fuertes, Gustavo,et al."Comment on "Innovative scattering analysis shows that hydrophobic disordered proteins are expanded in water"".SCIENCE 361.6405(2018). |
条目包含的文件 | 条目无相关文件。 |
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