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DOI | 10.1126/science.aar4199 |
Mediator and RNA polymerase II clusters associate in transcription-dependent condensates | |
Cho, Won-Ki1; Spille, Jan-Hendrik1; Hecht, Micca1; Lee, Choongman1; Li, Charles2,3; Grube, Valentin1,4; Cisse, Ibrahim I.1 | |
2018-07-27 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6400页码:412-415 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Germany |
英文摘要 | Models of gene control have emerged from genetic and biochemical studies, with limited consideration of the spatial organization and dynamics of key components in living cells. We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. Mediator and Poi II each form small transient and large stable clusters in living embryonic stem cells. Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. We suggest that large clusters of Mediator. recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000439923700045 |
WOS关键词 | SELECTIVE-INHIBITION ; SUPER-ENHANCERS ; HUMAN-CELLS ; MICROSCOPY ; LOCALIZATION ; ELEMENTS ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199249 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.MIT, Dept Phys, Cambridge, MA 02139 USA; 2.MIT, Dept Biol, Cambridge, MA 02139 USA; 3.Whitehead Inst Biomed Res, Cambridge, MA 02139 USA; 4.Ludwig Maximilians Univ Munchen, Dept Phys, Geschwister Scholl Pl 1, D-80539 Munich, Germany |
推荐引用方式 GB/T 7714 | Cho, Won-Ki,Spille, Jan-Hendrik,Hecht, Micca,et al. Mediator and RNA polymerase II clusters associate in transcription-dependent condensates[J]. SCIENCE,2018,361(6400):412-415. |
APA | Cho, Won-Ki.,Spille, Jan-Hendrik.,Hecht, Micca.,Lee, Choongman.,Li, Charles.,...&Cisse, Ibrahim I..(2018).Mediator and RNA polymerase II clusters associate in transcription-dependent condensates.SCIENCE,361(6400),412-415. |
MLA | Cho, Won-Ki,et al."Mediator and RNA polymerase II clusters associate in transcription-dependent condensates".SCIENCE 361.6400(2018):412-415. |
条目包含的文件 | 条目无相关文件。 |
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