Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aat5691 |
Three-dimensional intact-tissue sequencing of single-cell transcriptional states | |
Wang, Xiao1; Allen, William E.1,2; Wright, Matthew A.1,3; Sylwestrak, Emily L.1; Samusik, Nikolay4; Vesuna, Sam1; Evans, Kathryn1; Liu, Cindy1; Ramakrishnan, Charu1; Liu, Jia5; Nolan, Garry P.4; Bava, Felice-Alessio4,7; Deisseroth, Karl1,3,6 | |
2018-07-27 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 361期号:6400 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; France |
英文摘要 | Retrieving high-content gene-expression information while retaining three-dimensional (3D) positional anatomy at cellular resolution has been difficult, limiting integrative understanding of structure and function in complex biological tissues. We developed and applied a technology for 3D intact-tissue RNA sequencing, termed STARmap (spatially-resolved transcript amplicon readout mapping), which integrates hydrogel-tissue chemistry. targeted signal amplification, and in situ sequencing. The capabilities of STARmap were tested by mapping 160 to 1020 genes simultaneously in sections of mouse brain at single-cell resolution with high efficiency, accuracy, and reproducibility. Moving to thick tissue blocks, we observed a molecularly defined gradient distribution of excitatory-neuron subtypes across cubic millimeter-scale volumes (>30,000 cells) and a short-range 3D self-clustering in many inhibitory-neuron subtypes that could be identified and described with 3D STARmap. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000439923700038 |
WOS关键词 | RNA ; NEURONS ; COMMUNICATION ; HYBRIDIZATION ; INTERNEURONS ; EXPRESSION ; PRINCIPLES ; HYDROGELS ; NETWORKS ; PROTEINS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/199240 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA; 2.Stanford Univ, Neurosci Program, Stanford, CA 94305 USA; 3.Stanford Univ, Dept Psychiat & Behav Sci, Stanford, CA 94305 USA; 4.Stanford Univ, Dept Microbiol & Immunol, Baxter Lab, Stanford, CA 94305 USA; 5.Stanford Univ, Dept Chem Engn, Stanford, CA 94305 USA; 6.Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA; 7.Univ Paris Saclay, PSL Res Univ, Univ Paris Sud, Inst Curie,CNRS UMR 3348,Ctr Univ, F-91405 Orsay, France |
推荐引用方式 GB/T 7714 | Wang, Xiao,Allen, William E.,Wright, Matthew A.,et al. Three-dimensional intact-tissue sequencing of single-cell transcriptional states[J]. SCIENCE,2018,361(6400). |
APA | Wang, Xiao.,Allen, William E..,Wright, Matthew A..,Sylwestrak, Emily L..,Samusik, Nikolay.,...&Deisseroth, Karl.(2018).Three-dimensional intact-tissue sequencing of single-cell transcriptional states.SCIENCE,361(6400). |
MLA | Wang, Xiao,et al."Three-dimensional intact-tissue sequencing of single-cell transcriptional states".SCIENCE 361.6400(2018). |
条目包含的文件 | 条目无相关文件。 |
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