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DOI | 10.1126/science.aau0325 |
Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation | |
Bai, Rui1,2; Wan, Ruixue1,2; Yan, Chuangye1,2; Lei, Jianlin1,2,3; Shi, Yigong1,2,4 | |
2018-06-29 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6396页码:1423-1428 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
英文摘要 | The precatalytic spliceosome (B complex) is preceded by the pre-B complex. Here we report the cryo-electron microscopy structures of the Saccharomyces cerevisiae pre-B and B complexes at average resolutions of 3.3 to 4.6 and 3.9 angstroms, respectively. In the pre-B complex, the duplex between the 5' splice site (5' SS) and U1 small nuclear RNA (snRNA) is recognized by Yhc1, Luc7, and the Sm ring. In the B complex, U1 small nuclear ribonucleoprotein is dissociated, the 5'-exon-5'SS sequences are translocated near U6 snRNA, and three B-specific proteins may orient the precursor messenger RNA. In both complexes, U6 snRNA is anchored to loop I of U5 snRNA, and the duplex between the branch point sequence and U2 snRNA is recognized by the SF3b complex. Structural analysis reveals the mechanism of assembly and activation for the yeast spliceosome. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000436598000068 |
WOS关键词 | YEAST U1 SNRNP ; DEPENDENT ATPASE ACTIVITY ; U4/U6.U5 TRI-SNRNP ; GENETIC INTERACTIONS ; REQUIRES ATP ; RNA ; PROTEIN ; INSIGHTS ; SUBUNIT ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198984 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Sch Life Sci, Beijing 100084, Peoples R China; 2.Tsinghua Univ, Beijing Adv Innovat Ctr Struct Biol, Tsinghua Peking Joint Ctr Life Sci, Sch Med, Beijing 100084, Peoples R China; 3.Tsinghua Univ, Sch Life Sci, Technol Ctr Prot Sci, Minist Educ,Key Lab Prot Sci, Beijing 100084, Peoples R China; 4.Westlake Univ, Inst Biol, Westlake Inst Adv Study, 18 Shilongshan Rd, Hangzhou 310064, Zhejiang, Peoples R China |
推荐引用方式 GB/T 7714 | Bai, Rui,Wan, Ruixue,Yan, Chuangye,et al. Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation[J]. SCIENCE,2018,360(6396):1423-1428. |
APA | Bai, Rui,Wan, Ruixue,Yan, Chuangye,Lei, Jianlin,&Shi, Yigong.(2018).Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation.SCIENCE,360(6396),1423-1428. |
MLA | Bai, Rui,et al."Structures of the fully assembled Saccharomyces cerevisiae spliceosome before activation".SCIENCE 360.6396(2018):1423-1428. |
条目包含的文件 | 条目无相关文件。 |
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