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DOI | 10.1126/science.aat4100 |
Missing enzymes in the biosynthesis of the anticancer drug vinblastine in Madagascar periwinkle | |
Caputi, Lorenzo1; 39;Connor, Sarah E.2 | |
2018-06-15 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6394页码:1235-1238 |
文章类型 | Article |
语种 | 英语 |
国家 | England; France; Brazil; Germany |
英文摘要 | Vinblastine, a potent anticancer drug, is produced by Catharanthus roseus (Madagascar periwinkle) in small quantities, and heterologous reconstitution of vinblastine biosynthesis could provide an additional source of this drug. However, the chemistry underlying vinblastine synthesis makes identification of the biosynthetic genes challenging. Here we identify the two missing enzymes necessary for vinblastine biosynthesis in this plant: an oxidase and a reductase that isomerize stemmadenine acetate into dihydroprecondylocarpine acetate, which is then deacetoxylated and cyclized to either catharanthine or tabersonine via two hydrolases characterized herein. The pathways show how plants create chemical diversity and also enable development of heterologous platforms for generation of stemmadenine-derived bioactive compounds. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000435360400054 |
WOS关键词 | INDOLE ALKALOIDS ; STEREOSPECIFIC MODELS ; IBOGA-ALKALOIDS ; ASPIDOSPERMA ; PRECURSOR ; LOCALIZATION ; DIVERSITY ; CHEMISTRY ; BIOLOGY ; PATHWAY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198887 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.John Innes Ctr, Dept Biol Chem, Norwich Res Pk, Norwich NR4 7UH, Norfolk, England; 2.Univ Tours, Biomol & Biotechnol Vegetales, EA2106, Parc Grandmont, F-37200 Tours, France; 3.Univ Estadual N Fluminen, Lab Ciencias Quim, BR-28013602 Campos dos Goytacazes, RJ, Brazil; 4.Leibniz Univ Hannover, Ctr Biomol Drug Res, Schneiderberg 38, D-30167 Hannover, Germany; 5.Univ Oxford, Dept Biochem, South Parks Rd, Oxford OX1 3QU, England; 6.Univ Lancaster, Dept Biol, Lancaster LA1 4YW, England |
推荐引用方式 GB/T 7714 | Caputi, Lorenzo,39;Connor, Sarah E.. Missing enzymes in the biosynthesis of the anticancer drug vinblastine in Madagascar periwinkle[J]. SCIENCE,2018,360(6394):1235-1238. |
APA | Caputi, Lorenzo,&39;Connor, Sarah E..(2018).Missing enzymes in the biosynthesis of the anticancer drug vinblastine in Madagascar periwinkle.SCIENCE,360(6394),1235-1238. |
MLA | Caputi, Lorenzo,et al."Missing enzymes in the biosynthesis of the anticancer drug vinblastine in Madagascar periwinkle".SCIENCE 360.6394(2018):1235-1238. |
条目包含的文件 | 条目无相关文件。 |
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