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DOI | 10.1126/science.aar6436 |
Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface | |
Jenni, Simon1; Harrison, Stephen C.1,2 | |
2018-05-04 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6388页码:552-557 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule depolymerization to pull chromosomes apart during anaphase while resisting detachment as the microtubule shortens. The heterodecameric DASH/Dam1 complex (DASH/Dam1c), an essential component of yeast kinetochores, assembles into a microtubule-encircling ring. The ring associates with rodlike Ndc80 complexes to organize the kinetochore-microtubule interface. We report the cryo-electron microscopy structure (at similar to 4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis. Integrating this structure with that of the Ndc80 complex and with published interaction data yields a molecular picture of kinetochore-microtubule attachment, including how flexible, C-terminal extensions of DASH/Dam1c subunits project and contact widely separated sites on the Ndc80 complex rod and how phosphorylation at previously identified sites might regulate kinetochore assembly. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000431351500050 |
WOS关键词 | DAM1 ; RING ; DEPOLYMERIZATION ; ORGANIZATION ; ARCHITECTURE ; DRIVEN ; MOVEMENT ; SKA |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198570 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, 250 Longwood Ave, Boston, MA 02115 USA; 2.Harvard Univ, Howard Hughes Med Inst, 250 Longwood Ave, Boston, MA 02115 USA |
推荐引用方式 GB/T 7714 | Jenni, Simon,Harrison, Stephen C.. Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface[J]. SCIENCE,2018,360(6388):552-557. |
APA | Jenni, Simon,&Harrison, Stephen C..(2018).Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.SCIENCE,360(6388),552-557. |
MLA | Jenni, Simon,et al."Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface".SCIENCE 360.6388(2018):552-557. |
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