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DOI | 10.1126/science.aaq0179 |
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6 | |
Gootenberg, Jonathan S.1,2,3,4,5; Abudayyeh, Omar O.1,2,3,4,6; Kellner, Max J.1; Joung, Julia1,2,3,4; Collins, James J.1,4,6,7,8; Zhang, Feng1,2,3,4 | |
2018-04-27 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6387页码:439-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Rapid detection of nucleic acids is integral for clinical diagnostics and biotechnological applications. We recently developed a platform termed SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) that combines isothermal preamplification with Cas13 to detect single molecules of RNA or DNA. Through characterization of CRISPR enzymology and application development, we report here four advances integrated into SHERLOCK version 2 (SHERLOCKv2) (i) four-channel single-reaction multiplexing with orthogonal CRISPR enzymes; (ii) quantitative measurement of input as low as 2 attomolar; (iii) 3.5-fold increase in signal sensitivity by combining Cas13 with Csm6, an auxiliary CRISPR-associated enzyme; and (iv) lateral-flow readout. SHERLOCKv2 can detect Dengue or Zika virus single-stranded RNA as well as mutations in patient liquid biopsy samples via lateral flow, highlighting its potential as a multiplexable, portable, rapid, and quantitative detection platform of nucleic acids. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000430949600043 |
WOS关键词 | THERMOPHILUS ; CRISPR-CAS13 ; CLEAVAGE ; IMMUNITY ; SYSTEMS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198511 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Broad Inst Massachusetts Inst Technol MIT & Harva, Cambridge, MA 02142 USA; 2.MIT, McGovern Inst Brain Res, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 3.MIT, Dept Brain & Cognit Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 4.MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 5.Harvard Univ, Dept Syst Biol, Boston, MA 02115 USA; 6.MIT, Dept Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 7.MIT, Inst Med Engn & Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 8.Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA |
推荐引用方式 GB/T 7714 | Gootenberg, Jonathan S.,Abudayyeh, Omar O.,Kellner, Max J.,et al. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6[J]. SCIENCE,2018,360(6387):439-+. |
APA | Gootenberg, Jonathan S.,Abudayyeh, Omar O.,Kellner, Max J.,Joung, Julia,Collins, James J.,&Zhang, Feng.(2018).Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.SCIENCE,360(6387),439-+. |
MLA | Gootenberg, Jonathan S.,et al."Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6".SCIENCE 360.6387(2018):439-+. |
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