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DOI | 10.1126/science.aar1968 |
Asymmetric distribution and spatial switching of dynein activity generates ciliary motility | |
Lin, Jianfeng1,2; Nicastro, Daniela1,2 | |
2018-04-27 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6387 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Motile cilia and flagella are essential, highly conserved organelles, and their motility is driven by the coordinated activities of multiple dynein isoforms. The prevailing "switch-point" hypothesis posits that dyneins are asymmetrically activated to drive flagellar bending. To test this model, we applied cryo-electron tomography to visualize activity states of individual dyneins relative to their locations along beating flagella of sea urchin sperm cells. As predicted, bending was generated by the asymmetric distribution of dynein activity on opposite sides of the flagellum. However, contrary to predictions, most dyneins were in their active state, and the smaller population of conformationally inactive dyneins switched flagellar sides relative to the bending direction. Thus, our data suggest a "switch-inhibition" mechanism in which force imbalance is generated by inhibiting, rather than activating, dyneins on alternating sides of the flagellum. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000430949600031 |
WOS关键词 | INNER ARM DYNEIN ; IN-VIVO ; REGULATORY COMPLEX ; RADIAL SPOKES ; MECHANISM ; I1 ; FLAGELLA ; REVEALS ; PROTEIN ; VISUALIZATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198509 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Texas Southwestern Med Ctr Dallas, Dept Cell Biol & Biophys, Dallas, TX 75390 USA; 2.Brandeis Univ, Rosenstiel Basic Med Sci Res Ctr, Dept Biol, Waltham, MA 02454 USA |
推荐引用方式 GB/T 7714 | Lin, Jianfeng,Nicastro, Daniela. Asymmetric distribution and spatial switching of dynein activity generates ciliary motility[J]. SCIENCE,2018,360(6387). |
APA | Lin, Jianfeng,&Nicastro, Daniela.(2018).Asymmetric distribution and spatial switching of dynein activity generates ciliary motility.SCIENCE,360(6387). |
MLA | Lin, Jianfeng,et al."Asymmetric distribution and spatial switching of dynein activity generates ciliary motility".SCIENCE 360.6387(2018). |
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