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DOI | 10.1126/science.aar6245 |
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity | |
Chen, Janice S.1; Ma, Enbo1; Harrington, Lucas B.1; Da Costa, Maria2; Tian, Xinran3; Palefsky, Joel M.2; Doudna, Jennifer A.1,3,4,5,6 | |
2018-04-27 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 360期号:6387页码:436-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | CRISPR-Cas12a (Cpf1) proteins are RNA-guided enzymes that bind and cut DNA as components of bacterial adaptive immune systems. Like CRISPR-Cas9, Cas12a has been harnessed for genome editing on the basis of its ability to generate targeted, double-stranded DNA breaks. Here we show that RNA-guided DNA binding unleashes indiscriminate single-stranded DNA (ssDNA) cleavage activity by Cas12a that completely degrades ssDNA molecules. We find that target-activated, nonspecific single-stranded deoxyribonuclease (ssDNase) cleavage is also a property of other type V CRISPR-Cas12 enzymes. By combining Cas12a ssDNase activation with isothermal amplification, we create a method termed DNA endonuclease-targeted CRISPR trans reporter (DETECTR), which achieves attomolar sensitivity for DNA detection. DETECTR enables rapid and specific detection of human papillomavirus in patient samples, thereby providing a simple platform for molecular diagnostics. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000430949600042 |
WOS关键词 | CRISPR-CAS SYSTEMS ; HUMAN-CELLS ; CPF1 ; RNA ; ENDONUCLEASE ; CLEAVAGE ; SPECIFICITIES ; DIVERSITY ; EVOLUTION ; INFECTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198505 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA; 2.Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA; 3.Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA; 4.Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94704 USA; 5.Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA; 6.Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA |
推荐引用方式 GB/T 7714 | Chen, Janice S.,Ma, Enbo,Harrington, Lucas B.,et al. CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity[J]. SCIENCE,2018,360(6387):436-+. |
APA | Chen, Janice S..,Ma, Enbo.,Harrington, Lucas B..,Da Costa, Maria.,Tian, Xinran.,...&Doudna, Jennifer A..(2018).CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity.SCIENCE,360(6387),436-+. |
MLA | Chen, Janice S.,et al."CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity".SCIENCE 360.6387(2018):436-+. |
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