Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aaq1392 ; 10.1126/science.aaq1392 |
Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms; Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms | |
Liu, Tsung-Li1; Upadhyayula, Srigokul1,2,3,4; Milkie, Daniel E.1; Singh, Ved1; Wang, Kai1,12; Swinburne, Ian A.5; Mosaliganti, Kishore R.5; Collins, Zach M.5; Hiscock, Tom W.5; Shea, Jamien1; Kohrman, Abraham Q.6; Medwig, Taylor N.6; Dambournet, Daphne7; Forster, Ryan7; Cunniff, Brian2,3,13; Ruan, Yuan8,9; Yashiro, Hanako8,9; Scholpp, Steffen10,11; Meyerowitz, Elliot M.8,9; Hockemeyer, Dirk7; Drubin, David G.7; Martin, Benjamin L.6; Matus, David Q.6; Koyama, Minoru1; Megason, Sean G.5; Kirchhausen, Tom1,2,3,4; Betzig, Eric1 | |
2018-04-20 ; 2018-04-20 | |
发表期刊 | SCIENCE ; SCIENCE |
ISSN | 0036-8075 ; 0036-8075 |
EISSN | 1095-9203 ; 1095-9203 |
出版年 | 2018 ; 2018 |
卷号 | 360期号:6386 |
文章类型 | Article ; Article |
语种 | 英语 ; 英语 |
国家 | USA; USA; England; England; Germany; Germany; Peoples R China; Peoples R China |
英文摘要 | True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments. ; True physiological imaging of subcellular dynamics requires studying cells within their parent organisms, where all the environmental cues that drive gene expression, and hence the phenotypes that we actually observe, are present. A complete understanding also requires volumetric imaging of the cell and its surroundings at high spatiotemporal resolution, without inducing undue stress on either. We combined lattice light-sheet microscopy with adaptive optics to achieve, across large multicellular volumes, noninvasive aberration-free imaging of subcellular processes, including endocytosis, organelle remodeling during mitosis, and the migration of axons, immune cells, and metastatic cancer cells in vivo. The technology reveals the phenotypic diversity within cells across different organisms and developmental stages and may offer insights into how cells harness their intrinsic variability to adapt to different physiological environments. |
领域 | 地球科学 ; 地球科学 ; 气候变化 ; 气候变化 ; 资源环境 ; 资源环境 |
收录类别 | SCI-E ; SCI-E |
WOS记录号 | WOS:000430396600035 ; WOS:000430396600035 |
WOS关键词 | GROWTH CONES ; GROWTH CONES ; RESOLUTION ; RESOLUTION ; CLATHRIN ; CLATHRIN ; FILOPODIA ; FILOPODIA ; ENDOCYTOSIS ; ENDOCYTOSIS ; MICROSCOPY ; MICROSCOPY ; MIGRATION ; MIGRATION |
WOS类目 | Multidisciplinary Sciences ; Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics ; Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198462 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA; 2.Harvard Med Sch, Dept Cell Biol, 200 Longwood Ave, Boston, MA 02115 USA; 3.Boston Childrens Hosp, Program Cellular & Mol Med, 200 Longwood Ave, Boston, MA 02115 USA; 4.Harvard Med Sch, Dept Pediat, 200 Longwood Ave, Boston, MA 02115 USA; 5.Harvard Med Sch, Dept Syst Biol, 200 Longwood Ave, Boston, MA 02115 USA; 6.SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA; 7.Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA; 8.CALTECH, Howard Hughes Med Inst, Pasadena, CA 91125 USA; 9.CALTECH, Div Biol & Biol Engn, Pasadena, CA 91125 USA; 10.Univ Exeter, Coll Life & Environm Sci, Living Syst Inst, Exeter EX4 4QD, Devon, England; 11.Karlsruhe Inst Technol, Inst Toxicol & Genet, D-76021 Karlsruhe, Germany; 12.Chinese Acad Sci, Inst Neurosci, 320 Yueyang Rd, Shanghai, Peoples R China; 13.Univ Vermont, Coll Med, Dept Pathol, Burlington, VT 05401 USA |
推荐引用方式 GB/T 7714 | Liu, Tsung-Li,Upadhyayula, Srigokul,Milkie, Daniel E.,et al. Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms, Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms[J]. SCIENCE, SCIENCE,2018, 2018,360, 360(6386). |
APA | Liu, Tsung-Li.,Upadhyayula, Srigokul.,Milkie, Daniel E..,Singh, Ved.,Wang, Kai.,...&Betzig, Eric.(2018).Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms.SCIENCE,360(6386). |
MLA | Liu, Tsung-Li,et al."Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms".SCIENCE 360.6386(2018). |
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