Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aan0797 |
Mutation dynamics and fitness effects followed in single cells | |
Robert, Lydia1,2; Ollion, Jean1; Robert, Jerome1; Song, Xiaohu3; Matic, Ivan3; Elez, Marina1,4 | |
2018-03-16 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 359期号:6381页码:1283-1286 |
文章类型 | Article |
语种 | 英语 |
国家 | France |
英文摘要 | Mutations have been investigated for more than a century but remain difficult to observe directly in single cells, which limits the characterization of their dynamics and fitness effects. By combining microfluidics, time-lapse imaging, and a fluorescent tag of the mismatch repair system in Escherichia coli, we visualized the emergence of mutations in single cells, revealing Poissonian dynamics. Concomitantly, we tracked the growth and life span of single cells, accumulating similar to 20,000 mutations genome-wide over hundreds of generations. This analysis revealed that 1% of mutations were lethal; nonlethal mutations displayed a heavy-tailed distribution of fitness effects and were dominated by quasi-neutral mutations with an average cost of 0.3%. Our approach has enabled the investigation of single-cell individuality in mutation rate, mutation fitness costs, and mutation interactions. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000427504900047 |
WOS关键词 | BACTERIUM ESCHERICHIA-COLI ; DELETERIOUS MUTATIONS ; STRESS ; GENOME ; EPISTASIS ; EVOLUTION ; RATES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/198203 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.UPMC Univ Paris 06, Sorbonne Univ, UMR 8237, Lab Jean Perrin, Paris, France; 2.Univ Paris Saclay, AgroParisTech, INRA, Micalis Inst, Jouy En Josas, France; 3.Univ Paris 05, Fac Med Paris Descartes, Sorbonne Paris Cite, INSERM U1001, Paris, France; 4.Univ Paris Saclay, Univ Evry Val dEssonne, Inst Syst & Synthet Biol, UMR 8030,CNRS,Genopole,Commissariat Energie Atom, Evry, France |
推荐引用方式 GB/T 7714 | Robert, Lydia,Ollion, Jean,Robert, Jerome,et al. Mutation dynamics and fitness effects followed in single cells[J]. SCIENCE,2018,359(6381):1283-1286. |
APA | Robert, Lydia,Ollion, Jean,Robert, Jerome,Song, Xiaohu,Matic, Ivan,&Elez, Marina.(2018).Mutation dynamics and fitness effects followed in single cells.SCIENCE,359(6381),1283-1286. |
MLA | Robert, Lydia,et al."Mutation dynamics and fitness effects followed in single cells".SCIENCE 359.6381(2018):1283-1286. |
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