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DOI | 10.1126/science.aar1886 |
Lipopolysaccharide is transported to the cell surface by a membrane-to-membrane protein bridge | |
Sherman, David J.1; Xie, Ran1; Taylor, Rebecca. J.1; George, Alexander H.1; Okuda, Suguru1; Foster, Peter J.2,3,4; Needleman, Daniel J.2,3,4; Kahne, Daniel1,4,5 | |
2018-02-16 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 359期号:6377页码:798-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Gram-negative bacteria have an outer membrane that serves as a barrier to noxious agents in the environment. This protective function is dependent on lipopolysaccharide, a large glycolipid located in the outer leaflet of the outer membrane. Lipopolysaccharide is synthesized at the cytoplasmic membrane and must be transported to the cell surface. To understand this transport process, we reconstituted membrane-to-membrane movement of lipopolysaccharide by incorporating purified inner and outer membrane transport complexes into separate proteoliposomes. Transport involved stable association between the inner and outer membrane proteoliposomes. Our results support a model in which lipopolysaccharide molecules are pushed one after the other in a PEZ dispenser-like manner across a protein bridge that connects the inner and outer membranes. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000425116200046 |
WOS关键词 | BACTERIAL OUTER-MEMBRANE ; ESCHERICHIA-COLI ; SALMONELLA-TYPHIMURIUM ; FUNCTIONAL-ANALYSIS ; STRUCTURAL BASIS ; FLOW-CYTOMETRY ; CONTACT SITES ; COMPLEX ; EXPORT ; IDENTIFICATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197985 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA; 2.Harvard Univ, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA; 3.Harvard Univ, FAS Ctr Syst Biol, Cambridge, MA 02138 USA; 4.Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA; 5.Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA |
推荐引用方式 GB/T 7714 | Sherman, David J.,Xie, Ran,Taylor, Rebecca. J.,et al. Lipopolysaccharide is transported to the cell surface by a membrane-to-membrane protein bridge[J]. SCIENCE,2018,359(6377):798-+. |
APA | Sherman, David J..,Xie, Ran.,Taylor, Rebecca. J..,George, Alexander H..,Okuda, Suguru.,...&Kahne, Daniel.(2018).Lipopolysaccharide is transported to the cell surface by a membrane-to-membrane protein bridge.SCIENCE,359(6377),798-+. |
MLA | Sherman, David J.,et al."Lipopolysaccharide is transported to the cell surface by a membrane-to-membrane protein bridge".SCIENCE 359.6377(2018):798-+. |
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