Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aao6326 |
Fatty acyl recognition and transfer by an integral membrane S-acyltransferase | |
Rana, Mitra S.1; Kumar, Pramod1; Lee, Chul-Jin1; Verardi, Raffaello1; Rajashankar, Kanagalaghatta R.2,3; Banerjee, Anirban1 | |
2018-01-12 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2018 |
卷号 | 359期号:6372页码:176-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | DHHC (Asp-His-His-Cys) palmitoyltransferases are eukaryotic integral membrane enzymes that catalyze protein palmitoylation, which is important in a range of physiological processes, including small guanosine triphosphatase (GTPase) signaling, cell adhesion, and neuronal receptor scaffolding. We present crystal structures of two DHHC palmitoyltransferases and a covalent intermediate mimic. The active site resides at the membrane-cytosol interface, which allows the enzyme to catalyze thioester-exchange chemistry by using fatty acyl-coenzyme A and explains why membrane-proximal cysteines are candidates for palmitoylation. The acyl chain binds in a cavity formed by the transmembrane domain. We propose a mechanism for acyl chain-length selectivity in DHHC enzymes on the basis of cavity mutants with preferences for shorter and longer acyl chains. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000419816600038 |
WOS关键词 | CYSTEINE-RICH DOMAIN ; PALMITOYL ACYLTRANSFERASE ; DUAL PALMITOYLATION ; ACYLATION ; PROTEINS ; ACID ; LOCALIZATION ; PALMITOYLTRANSFERASE ; IDENTIFICATION ; SELECTIVITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197746 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.NICHHD, Cell Biol & Neurobiol Branch, NIH, Bethesda, MD 20892 USA; 2.Cornell Univ, Argonne Natl Lab, Northeastern Collaborat Access Team NE CAT, Bldg 436E,9700 South Cass Ave, Argonne, IL 60439 USA; 3.Cornell Univ, Argonne Natl Lab, Dept Chem & Chem Biol, Bldg 436E,9700 South Cass Ave, Argonne, IL 60439 USA |
推荐引用方式 GB/T 7714 | Rana, Mitra S.,Kumar, Pramod,Lee, Chul-Jin,et al. Fatty acyl recognition and transfer by an integral membrane S-acyltransferase[J]. SCIENCE,2018,359(6372):176-+. |
APA | Rana, Mitra S.,Kumar, Pramod,Lee, Chul-Jin,Verardi, Raffaello,Rajashankar, Kanagalaghatta R.,&Banerjee, Anirban.(2018).Fatty acyl recognition and transfer by an integral membrane S-acyltransferase.SCIENCE,359(6372),176-+. |
MLA | Rana, Mitra S.,et al."Fatty acyl recognition and transfer by an integral membrane S-acyltransferase".SCIENCE 359.6372(2018):176-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论