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DOI | 10.1126/science.aap9221 |
Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2 | |
Aigner, H.; Wilson, R. H.; Bracher, A.; Calisse, L.; Bhat, J. Y.; Hartl, F. U.; Hayer-Hartl, M. | |
2017-12-08 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6368页码:1272-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | Plant RuBisCo, a complex of eight large and eight small subunits, catalyzes the fixation of CO2 in photosynthesis. The low catalytic efficiency of RuBisCo provides strong motivation to reengineer the enzyme with the goal of increasing crop yields. However, genetic manipulation has been hampered by the failure to express plant RuBisCo in a bacterial host. We achieved the functional expression of Arabidopsis thaliana RuBisCo in Escherichia coli by coexpressing multiple chloroplast chaperones. These include the chaperonins Cpn60/Cpn20, RuBisCo accumulation factors 1 and 2, RbcX, and bundle-sheath defective-2 (BSD2). Our structural and functional analysis revealed the role of BSD2 in stabilizing an end-state assembly intermediate of eight RuBisCo large subunits until the small subunits become available. The ability to produce plant RuBisCo recombinantly will facilitate efforts to improve the enzyme through mutagenesis. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000417254700052 |
WOS关键词 | RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE ; TEMPERATURE RESPONSE ; RBCX ; PROTEIN ; PHOTOSYNTHESIS ; BIOGENESIS ; PROSPECTS ; GENE ; EXPRESSION ; FUTURE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197487 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | Max Planck Inst Biochem, Dept Cellular Biochem, Martinsried, Germany |
推荐引用方式 GB/T 7714 | Aigner, H.,Wilson, R. H.,Bracher, A.,et al. Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2[J]. SCIENCE,2017,358(6368):1272-+. |
APA | Aigner, H..,Wilson, R. H..,Bracher, A..,Calisse, L..,Bhat, J. Y..,...&Hayer-Hartl, M..(2017).Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2.SCIENCE,358(6368),1272-+. |
MLA | Aigner, H.,et al."Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2".SCIENCE 358.6368(2017):1272-+. |
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