Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aam9305 |
Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass | |
Spinelli, Jessica B.1,2; Yoon, Haejin1; Ringel, Alison E.1; Jeanfavre, Sarah2; Clish, Clary B.2; Haigis, Marcia C.1 | |
2017-11-17 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6365页码:941-946 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Ammonia is a ubiquitous by-product of cellular metabolism; however, the biological consequences of ammonia production are not fully understood, especially in cancer. We found that ammonia is not merely a toxic waste product but is recycled into central amino acid metabolism to maximize nitrogen utilization. In our experiments, human breast cancer cells primarily assimilated ammonia through reductive amination catalyzed by glutamate dehydrogenase (GDH); secondary reactions enabled other amino acids, such as proline and aspartate, to directly acquire this nitrogen. Metabolic recycling of ammonia accelerated proliferation of breast cancer. In mice, ammonia accumulated in the tumor microenvironment and was used directly to generate amino acids through GDH activity. These data show that ammonia is not only a secreted waste product but also a fundamental nitrogen source that can support tumor biomass. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000415293000051 |
WOS关键词 | UREA CYCLE ; PROLIFERATION ; BIOSYNTHESIS ; MITOCHONDRIA ; GLUTAMINASE ; SYNTHETASE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197354 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Harvard Med Sch, Dept Cell Biol, Boston, MA 02115 USA; 2.Broad Inst MIT & Harvard, Cambridge, MA 02142 USA |
推荐引用方式 GB/T 7714 | Spinelli, Jessica B.,Yoon, Haejin,Ringel, Alison E.,et al. Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass[J]. SCIENCE,2017,358(6365):941-946. |
APA | Spinelli, Jessica B.,Yoon, Haejin,Ringel, Alison E.,Jeanfavre, Sarah,Clish, Clary B.,&Haigis, Marcia C..(2017).Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass.SCIENCE,358(6365),941-946. |
MLA | Spinelli, Jessica B.,et al."Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass".SCIENCE 358.6365(2017):941-946. |
条目包含的文件 | 条目无相关文件。 |
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