Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aan3235 |
The Drosophila embryo at single-cell transcriptome resolution | |
Karaiskos, Nikos1; Wahle, Philipp2; Alles, Jonathan1; Boltengagen, Anastasiya1; Ayoub, Salah1; Kipar, Claudia2; Kocks, Christine1; Rajewsky, Nikolaus1; Zinzen, Robert P.2 | |
2017-10-13 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 358期号:6360页码:194-199 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | By the onset of morphogenesis, Drosophila embryos consist of about 6000 cells that express distinct gene combinations. Here, we used single-cell sequencing of precisely staged embryos and devised DistMap, a computational mapping strategy to reconstruct the embryo and to predict spatial gene expression approaching single-cell resolution. We produced a virtual embryo with about 8000 expressed genes per cell. Our interactive Drosophila Virtual Expression eXplorer (DVEX) database generates three-dimensional virtual in situ hybridizations and computes gene expression gradients. We used DVEX to uncover patterned expression of transcription factors and long noncoding RNAs, as well as signaling pathway components. Spatial regulation of Hippo signaling during early embryogenesis suggests a mechanism for establishing asynchronous cell proliferation. Our approach is suitable to generate transcriptomic blueprints for other complex tissues. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000412841500041 |
WOS关键词 | GENOME-WIDE EXPRESSION ; GENE-EXPRESSION ; RNA-SEQ ; IN-VIVO ; MELANOGASTER ; REVEALS ; FATE ; LOCALIZATION ; ACTIVATION ; EVOLUTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/197098 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Helmholtz Assoc MDC, Berlin Max Delbruck Ctr Mol Med, Syst Biol Gene Regulatory Elements, D-13125 Berlin, Germany; 2.MDC, Syst Biol Neural Tissue Differentiat, BIMSB, D-13125 Berlin, Germany |
推荐引用方式 GB/T 7714 | Karaiskos, Nikos,Wahle, Philipp,Alles, Jonathan,et al. The Drosophila embryo at single-cell transcriptome resolution[J]. SCIENCE,2017,358(6360):194-199. |
APA | Karaiskos, Nikos.,Wahle, Philipp.,Alles, Jonathan.,Boltengagen, Anastasiya.,Ayoub, Salah.,...&Zinzen, Robert P..(2017).The Drosophila embryo at single-cell transcriptome resolution.SCIENCE,358(6360),194-199. |
MLA | Karaiskos, Nikos,et al."The Drosophila embryo at single-cell transcriptome resolution".SCIENCE 358.6360(2017):194-199. |
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