Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

doi:10.1126/science.aan4187

GSTDTAP > 地球科学

DOI	10.1126/science.aan4187
	Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9
	Niu, Dong 1,2; Wei, Hong-Jiang 3,4; Lin, Lin 5; George, Haydy 1; Wang, Tao 1; Lee, Hsiu 1; Zhao, Hong-Ye 3; Wang, Yong 6; Kan, Yinan 1; Shrock, Ellen 7; Lesha, Emal 1; Wang, Gang 1; Luo, Yonglun 5; Qing, Yubo 3,4; Jiao, Deling 3,4; Zhao, Heng 3,4; Zhou, Xiaoyang 6; Wang, Shouqi 8; Wei, Hong 6; Gueell, Marc 1; Church, George M.1,7,9; Yang, Luhan 1
	2017-09-22
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2017
卷号	357 期号:6357 页码:1303-1307
文章类型	Article
语种	英语
国家	USA; Peoples R China; Denmark
英文摘要	Xenotransplantation is a promising strategy to alleviate the shortage of organs for human transplantation. In addition to the concerns about pig-to-human immunological compatibility, the risk of cross-species transmission of porcine endogenous retroviruses (PERVs) has impeded the clinical application of this approach. We previously demonstrated the feasibility of inactivating PERV activity in an immortalized pig cell line. We now confirm that PERVs infect human cells, and we observe the horizontal transfer of PERVs among human cells. Using CRISPR-Cas9, we inactivated all of the PERVs in a porcine primary cell line and generated PERV-inactivated pigs via somatic cell nuclear transfer. Our study highlights the value of PERV inactivation to prevent cross-species viral transmission and demonstrates the successful production of PERV-inactivated animals to address the safety concern in clinical xenotransplantation.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000411337700048
WOS关键词	INFECTION ; GENOMES ; CELLS ; PERVS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/196923
专题	地球科学资源环境科学气候变化
作者单位	1.eGenesis, Cambridge, MA 02139 USA; 2.Zhejiang Univ, Coll Anim Sci, Hangzhou 310058, Zhejiang, Peoples R China; 3.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources, Kunming 650201, Yunnan, Peoples R China; 4.Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Yunnan, Peoples R China; 5.Aarhus Univ, Dept Biomed, DK-8000 Aarhus C, Denmark; 6.Third Mil Med Univ, Dept Lab Anim Sci, Coll Basic Med Sci, Chongqing 400038, Peoples R China; 7.Harvard Med Sch, Dept Genet, Boston, MA 02115 USA; 8.Res Inst Shenzhen Jinxinnong Technol, Shenzhen 518106, Peoples R China; 9.Harvard Univ, Wyss Inst Biol Inspired Engn, Cambridge, MA 02138 USA
推荐引用方式 GB/T 7714	Niu, Dong,Wei, Hong-Jiang,Lin, Lin,et al. Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9[J]. SCIENCE,2017,357(6357):1303-1307.
APA	Niu, Dong.,Wei, Hong-Jiang.,Lin, Lin.,George, Haydy.,Wang, Tao.,...&Yang, Luhan.(2017).Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9.SCIENCE,357(6357),1303-1307.
MLA	Niu, Dong,et al."Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9".SCIENCE 357.6357(2017):1303-1307.