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DOI | 10.1126/science.aao0679 |
Structures of the CRISPR genome integration complex | |
Wright, Addison V.1; Liu, Jun-Jie1,2; Knott, Gavin J.1; Doxzen, Kevin W.3; Nogales, Eva1,2,4; Doudna, Jennifer A.1,2,3,4,5,6,7 | |
2017-09-15 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 357期号:6356页码:1113-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | CRISPR-Cas systems depend on the Cas1-Cas2 integrase to capture and integrate short foreign DNA fragments into the CRISPR locus, enabling adaptation to new viruses. We present crystal structures of Cas1-Cas2 bound to both donor and target DNA in intermediate and product integration complexes, as well as a cryo-electron microscopy structure of the full CRISPR locus integration complex, including the accessory protein IHF (integration host factor). The structures show unexpectedly that indirect sequence recognition dictates integration site selection by favoring deformation of the repeat and the flanking sequences. IHF binding bends the DNA sharply, bringing an upstream recognition motif into contact with Cas1 to increase both the specificity and efficiency of integration. These results explain how the Cas1-Cas2 CRISPR integrase recognizes a sequence-dependent DNA structure to ensure site-selective CRISPR array expansion during the initial step of bacterial adaptive immunity. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000410639800035 |
WOS关键词 | SPACER ACQUISITION ; HOST FACTOR ; DNA ; PROTEIN ; ADAPTATION ; REPEATS ; SITE ; PROTOSPACER ; ELEMENTS ; CAPTURE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196869 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif Berkeley, Dept Mol & Cell Biol, 229 Stanley Hall, Berkeley, CA 94720 USA; 2.Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA; 3.Univ Calif Berkeley, Biophys Grad Grp, Berkeley, CA 94720 USA; 4.Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA; 5.Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA; 6.Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94720 USA; 7.Univ Calif Berkeley, Ctr RNA Syst Biol, Berkeley, CA 94720 USA |
推荐引用方式 GB/T 7714 | Wright, Addison V.,Liu, Jun-Jie,Knott, Gavin J.,et al. Structures of the CRISPR genome integration complex[J]. SCIENCE,2017,357(6356):1113-+. |
APA | Wright, Addison V.,Liu, Jun-Jie,Knott, Gavin J.,Doxzen, Kevin W.,Nogales, Eva,&Doudna, Jennifer A..(2017).Structures of the CRISPR genome integration complex.SCIENCE,357(6356),1113-+. |
MLA | Wright, Addison V.,et al."Structures of the CRISPR genome integration complex".SCIENCE 357.6356(2017):1113-+. |
条目包含的文件 | 条目无相关文件。 |
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