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DOI | 10.1126/science.aam8940 |
Comprehensive single-cell transcriptional profiling of a multicellular organism | |
Cao, Junyue1,2; Packer, Jonathan S.1; Ramani, Vijay1; Cusanovich, Darren A.1; Huynh, Chau1; Daza, Riza1; Qiu, Xiaojie1,2; Lee, Choli1; Furlan, Scott N.3,4,5; Steemers, Frank J.6; Adey, Andrew7,8; Waterston, Robert H.1; Trapnell, Cole1; Shendure, Jay1,9 | |
2017-08-18 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 357期号:6352页码:661-667 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | To resolve cellular heterogeneity, we developed a combinatorial indexing strategy to profile the transcriptomes of single cells or nuclei, termed sci-RNA-seq (single-cell combinatorial indexing RNA sequencing). We applied sci-RNA-seq to profile nearly 50,000 cells from the nematode Caenorhabditis elegans at the L2 larval stage, which provided >50-fold "shotgun" cellular coverage of its somatic cell composition. From these data, we defined consensus expression profiles for 27 cell types and recovered rare neuronal cell types corresponding to as few as one or two cells in the L2 worm. We integrated these profiles with whole-animal chromatin immunoprecipitation sequencing data to deconvolve the cell type-specific effects of transcription factors. The data generated by sci-RNA-seq constitute a powerful resource for nematode biology and foreshadow similar atlases for other organisms. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000407793600029 |
WOS关键词 | NEMATODE CAENORHABDITIS-ELEGANS ; MESSENGER-RNA-SEQ ; GENE-EXPRESSION ; NERVOUS-SYSTEM ; GENOME ; CONTIGUITY ; PATHWAYS ; CORTEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196694 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA; 2.Univ Washington, Mol & Cellular Biol Program, Seattle, WA 98195 USA; 3.Seattle Childrens Res Inst, Ben Towne Ctr Childhood Canc Res, Seattle, WA USA; 4.Univ Washington, Dept Pediat, Seattle, WA 98195 USA; 5.Fred Hutchinson Canc Res Ctr, 1124 Columbia St, Seattle, WA 98104 USA; 6.Adv Res Grp, San Diego, CA USA; 7.Oregon Hlth & Sci Univ, Dept Mol & Med Genet, Portland, OR 97201 USA; 8.Knight Cardiovasc Inst, Portland, OR USA; 9.Howard Hughes Med Inst, Seattle, WA 02115 USA |
推荐引用方式 GB/T 7714 | Cao, Junyue,Packer, Jonathan S.,Ramani, Vijay,et al. Comprehensive single-cell transcriptional profiling of a multicellular organism[J]. SCIENCE,2017,357(6352):661-667. |
APA | Cao, Junyue.,Packer, Jonathan S..,Ramani, Vijay.,Cusanovich, Darren A..,Huynh, Chau.,...&Shendure, Jay.(2017).Comprehensive single-cell transcriptional profiling of a multicellular organism.SCIENCE,357(6352),661-667. |
MLA | Cao, Junyue,et al."Comprehensive single-cell transcriptional profiling of a multicellular organism".SCIENCE 357.6352(2017):661-667. |
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