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DOI | 10.1126/science.aan0425 |
Methanogenic heterodisulfide reductase (HdrABC-MvhAGD) uses two noncubane [4Fe-4S] clusters for reduction | |
Wagner, Tristan1; Koch, Juergen1; Ermler, Ulrich2; Shima, Seigo1 | |
2017-08-18 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 357期号:6352页码:699-702 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany |
英文摘要 | In methanogenic archaea, the carbon dioxide (CO2) fixation and methane-forming steps are linked through the heterodisulfide reductase (HdrABC)-[NiFe]-hydrogenase (MvhAGD) complex that uses flavin-based electron bifurcation to reduce ferredoxin and the heterodisulfide of coenzymes M and B. Here, we present the structure of the native heterododecameric HdrABC-MvhAGD complex at 2.15-angstrom resolution. HdrB contains two noncubane [4Fe-4S] clusters composed of fused [3Fe-4S]-[2Fe-2S] units sharing 1 iron (Fe) and 1 sulfur (S), which were coordinated at the CCG motifs. Soaking experiments showed that the heterodisulfide is clamped between the two noncubane [4Fe-4S] clusters and homolytically cleaved, forming coenzyme M and B bound to each iron. Coenzymes are consecutively released upon one-by-one electron transfer. The HdrABC-MvhAGD atomic model serves as a structural template for numerous HdrABC homologs involved in diverse microbial metabolic pathways. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000407793600038 |
WOS关键词 | METHANOBACTERIUM-THERMOAUTOTROPHICUM ; ELECTRON BIFURCATION ; OXIDOREDUCTASE ; CONTAINS ; INSIGHTS ; ARCHAEA ; FERREDOXIN ; MECHANISM ; PATHWAY ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196690 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Max Planck Inst Terr Microbiol, Karl von Frisch Str 10, D-35043 Marburg, Germany; 2.Max Planck Inst Biophys, Max von Laue Str 3, D-60438 Frankfurt, Germany |
推荐引用方式 GB/T 7714 | Wagner, Tristan,Koch, Juergen,Ermler, Ulrich,et al. Methanogenic heterodisulfide reductase (HdrABC-MvhAGD) uses two noncubane [4Fe-4S] clusters for reduction[J]. SCIENCE,2017,357(6352):699-702. |
APA | Wagner, Tristan,Koch, Juergen,Ermler, Ulrich,&Shima, Seigo.(2017).Methanogenic heterodisulfide reductase (HdrABC-MvhAGD) uses two noncubane [4Fe-4S] clusters for reduction.SCIENCE,357(6352),699-702. |
MLA | Wagner, Tristan,et al."Methanogenic heterodisulfide reductase (HdrABC-MvhAGD) uses two noncubane [4Fe-4S] clusters for reduction".SCIENCE 357.6352(2017):699-702. |
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