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DOI | 10.1126/science.aam7787 |
CAT-tailing as a fail-safe mechanism for efficient degradation of stalled nascent polypeptides | |
Kostova, Kamena K.1,2; Hickey, Kelsey L.1; Osuna, Beatriz A.1,3; Hussmann, Jeffrey A.4; Frost, Adam3,5,6,7; Weinberg, David E.1; Weissman, Jonathan S.1,2,5,8,9 | |
2017-07-28 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 357期号:6349页码:414-417 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Ribosome stalling leads to recruitment of the ribosome quality control complex (RQC), which targets the partially synthesized polypeptide for proteasomal degradation through the action of the ubiquitin ligase Ltn1p. A second core RQC component, Rqc2p, modifies the nascent polypeptide by adding a carboxyl-terminal alanine and threonine (CAT) tail through a noncanonical elongation reaction. Here we examined the role of CAT-tailing in nascent-chain degradation in budding yeast. We found that Ltn1p efficiently accessed only nascent-chain lysines immediately proximal to the ribosome exit tunnel. For substrates without Ltn1p-accessible lysines, CAT-tailing enabled degradation by exposing lysines sequestered in the ribosome exit tunnel. Thus, CAT-tails do not serve as a degron, but rather provide a fail-safe mechanism that expands the range of RQC-degradable substrates. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000406362300048 |
WOS关键词 | PROTEIN-QUALITY CONTROL ; LARGE RIBOSOMAL-SUBUNIT ; MESSENGER-RNA ; STRUCTURAL BASIS ; CONTROL COMPLEX ; TRANSLATION ; UBIQUITINATION ; SURVEILLANCE ; AGGREGATION ; PROTEASOME |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196556 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif San Francisco, Dept Cellular & Mol Pharmacol, San Francisco, CA 94158 USA; 2.Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94158 USA; 3.Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA; 4.Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94158 USA; 5.Univ Calif San Francisco, Calif Inst Quantitat Biomed Res, San Francisco, CA 94158 USA; 6.Univ Utah, Dept Biochem, Salt Lake City, UT 84112 USA; 7.Chan Zuckerberg Biohub, San Francisco, CA 94158 USA; 8.Univ Calif San Francisco, Ctr RNA Syst Biol, San Francisco, CA 94158 USA; 9.Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94720 USA |
推荐引用方式 GB/T 7714 | Kostova, Kamena K.,Hickey, Kelsey L.,Osuna, Beatriz A.,et al. CAT-tailing as a fail-safe mechanism for efficient degradation of stalled nascent polypeptides[J]. SCIENCE,2017,357(6349):414-417. |
APA | Kostova, Kamena K..,Hickey, Kelsey L..,Osuna, Beatriz A..,Hussmann, Jeffrey A..,Frost, Adam.,...&Weissman, Jonathan S..(2017).CAT-tailing as a fail-safe mechanism for efficient degradation of stalled nascent polypeptides.SCIENCE,357(6349),414-417. |
MLA | Kostova, Kamena K.,et al."CAT-tailing as a fail-safe mechanism for efficient degradation of stalled nascent polypeptides".SCIENCE 357.6349(2017):414-417. |
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