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DOI | 10.1126/science.aaj1669 |
Local protein kinase A action proceeds through intact holoenzymes | |
Smith, F. Donelson1; Esseltine, Jessica L.1,5; Nygren, Patrick J.1; Veesler, David2; Byrne, Dominic P.3; Vonderach, Matthias3; Strashnov, Ilya4; Eyers, Claire E.3; Eyers, Patrick A.3; Langeberg, Lorene K.1; Scott, John D.1 | |
2017-06-23 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 356期号:6344页码:1288-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA; England; Canada |
英文摘要 | Hormones can transmit signals through adenosine 3',5'-monophosphate (cAMP) to precise intracellular locations. The fidelity of these responses relies on the activation of localized protein kinase A (PKA) holoenzymes. Association of PKA regulatory type II (RII) subunits with A-kinase-anchoring proteins (AKAPs) confers location, and catalytic (C) subunits phosphorylate substrates. Single-particle electron microscopy demonstrated that AKAP79 constrains RII-C subassemblies within 150 to 250 angstroms of its targets. Native mass spectrometry established that these macromolecular assemblies incorporated stoichiometric amounts of cAMP. Chemical-biology-and live cell-imaging techniques revealed that catalytically active PKA holoenzymes remained intact within the cytoplasm. These findings indicate that the parameters of anchored PKA holoenzyme action are much more restricted than originally anticipated. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000403881700041 |
WOS关键词 | MASS-SPECTROMETRY ; SKELETAL-MUSCLE ; CAMP ; PHOSPHORYLATION ; PKA ; BAD ; INHIBITION ; ACTIVATION ; SUBUNIT ; COMPLEX |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196321 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Washington, Howard Hughes Med Inst, Dept Pharmacol, Seattle, WA 98195 USA; 2.Univ Washington, Dept Biochem, Seattle, WA 98195 USA; 3.Univ Liverpool, Inst Integrat Biol, Dept Biochem, Liverpool L69 7ZB, Merseyside, England; 4.Univ Manchester, Sch Chem, Manchester M13 9PL, Lancs, England; 5.Western Univ, Schulich Sch Med & Dent, London, ON N6A 5C1, Canada |
推荐引用方式 GB/T 7714 | Smith, F. Donelson,Esseltine, Jessica L.,Nygren, Patrick J.,et al. Local protein kinase A action proceeds through intact holoenzymes[J]. SCIENCE,2017,356(6344):1288-+. |
APA | Smith, F. Donelson.,Esseltine, Jessica L..,Nygren, Patrick J..,Veesler, David.,Byrne, Dominic P..,...&Scott, John D..(2017).Local protein kinase A action proceeds through intact holoenzymes.SCIENCE,356(6344),1288-+. |
MLA | Smith, F. Donelson,et al."Local protein kinase A action proceeds through intact holoenzymes".SCIENCE 356.6344(2017):1288-+. |
条目包含的文件 | 条目无相关文件。 |
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