Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aal3118 |
Visualizing dynamic microvillar search and stabilization during ligand detection by T cells | |
Cai, En1; Marchuk, Kyle1,2; Beemiller, Peter1; Beppler, Casey1; Rubashkin, Matthew G.3; Weaver, Valerie M.3; Gerard, Audrey1; Liu, Tsung-Li4; Chen, Bi-Chang4; Betzig, Eric4; Bartumeus, Frederic5,6,7; Krummel, Matthew F.1,2 | |
2017-05-12 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 356期号:6338 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Spain |
英文摘要 | During immune surveillance, T cells survey the surface of antigen-presenting cells. In searching for peptide-loaded major histocompatibility complexes (pMHCs), they must solve a classic trade-off between speed and sensitivity. It has long been supposed that microvilli on T cells act as sensory organs to enable search, but their strategy has been unknown. We used lattice light-sheet and quantum dot-enabled synaptic contact mapping microscopy to show that anomalous diffusion and fractal organization of microvilli survey the majority of opposing surfaces within 1 minute. Individual dwell times were long enough to discriminate pMHC half-lives and T cell receptor (TCR) accumulation selectively stabilized microvilli. Stabilization was independent of tyrosine kinase signaling and the actin cytoskeleton, suggesting selection for avid TCR microclusters. This work defines the efficient cellular search process against which ligand detection takes place. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000400975200046 |
WOS关键词 | IMMUNOLOGICAL SYNAPSE ; ADHESION ; MICROCLUSTERS ; MOTILITY ; SEGREGATION ; RECEPTORS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/196022 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Calif San Francisco, Dept Pathol, San Francisco, CA 94143 USA; 2.Univ Calif San Francisco, Biol Imaging Dev Ctr, San Francisco, CA 94143 USA; 3.Univ Calif San Francisco, Ctr Bioengn & Tissue Regenerat, San Francisco, CA 94143 USA; 4.Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA; 5.Ctr Adv Studies Blanes CEAB CSIC, Girona 17300, Spain; 6.Ecol & Forestry Applicat Res Ctr CREAF, Barcelona 08193, Spain; 7.Inst Catala Recerca & Estudis Avancats, Barcelona 08010, Spain |
推荐引用方式 GB/T 7714 | Cai, En,Marchuk, Kyle,Beemiller, Peter,et al. Visualizing dynamic microvillar search and stabilization during ligand detection by T cells[J]. SCIENCE,2017,356(6338). |
APA | Cai, En.,Marchuk, Kyle.,Beemiller, Peter.,Beppler, Casey.,Rubashkin, Matthew G..,...&Krummel, Matthew F..(2017).Visualizing dynamic microvillar search and stabilization during ligand detection by T cells.SCIENCE,356(6338). |
MLA | Cai, En,et al."Visualizing dynamic microvillar search and stabilization during ligand detection by T cells".SCIENCE 356.6338(2017). |
条目包含的文件 | 条目无相关文件。 |
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