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DOI | 10.1126/science.aaj2239 |
Impact of cytosine methylation on DNA binding specificities of human transcription factors | |
Yin, Yimeng1; Morgunova, Ekaterina1; Jolma, Arttu1; Kaasinen, Eevi1; Sahu, Biswajyoti2; Khund-Sayeed, Syed3; Das, Pratyush K.2; Kivioja, Teemu2; Dave, Kashyap1; Zhong, Fan1; Nitta, Kazuhiro R.1; Taipale, Minna1; Popov, Alexander4; Ginno, Paul A.5; Domcke, Silvia5,6; Yan, Jian1; Schubeler, Dirk5,6; Vinson, Charles3; Taipale, Jussi1,2 | |
2017-05-05 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 356期号:6337 |
文章类型 | Article |
语种 | 英语 |
国家 | Sweden; Finland; USA; France; Switzerland |
英文摘要 | The majority of CpG dinucleotides in the human genome are methylated at cytosine bases. However, active gene regulatory elements are generally hypomethylated relative to their flanking regions, and the binding of some transcription factors (TFs) is diminished by methylation of their target sequences. By analysis of 542 human TFs with methylation-sensitive SELEX (systematic evolution of ligands by exponential enrichment), we found that there are also many TFs that prefer CpG-methylated sequences. Most of these are in the extended homeodomain family. Structural analysis showed that homeodomain specificity for methylcytosine depends on direct hydrophobic interactions with the methylcytosine 5-methyl group. This study provides a systematic examination of the effect of an epigenetic DNA modification on human TF binding specificity and reveals that many developmentally important proteins display preference for mCpG-containing sequences. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000400545700029 |
WOS关键词 | EMBRYONIC STEM-CELLS ; CPG METHYLATION ; GENE-EXPRESSION ; SEQUENCING DATA ; READ ALIGNMENT ; RECOGNITION ; CHROMATIN ; PROTEINS ; PROMOTER ; DOMAIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195986 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Karolinska Inst, Dept Med Biochem & Biophys, Div Funct Genom & Syst Biol, SE-14183 Stockholm, Sweden; 2.Univ Helsinki, Genome Scale Biol Program, POB 63, FI-00014 Helsinki, Finland; 3.NCI, Lab Metab, NIH, Room 3128,Bldg 37, Bethesda, MD 20892 USA; 4.European Synchrotron Radiat Facil, F-38043 Grenoble, France; 5.Friedrich Miescher Inst Biomed Res FMI, Maulbeerstr 66, CH-4058 Basel, Switzerland; 6.Univ Basel, Fac Sci, Peterspl 1, CH-4003 Basel, Switzerland |
推荐引用方式 GB/T 7714 | Yin, Yimeng,Morgunova, Ekaterina,Jolma, Arttu,et al. Impact of cytosine methylation on DNA binding specificities of human transcription factors[J]. SCIENCE,2017,356(6337). |
APA | Yin, Yimeng.,Morgunova, Ekaterina.,Jolma, Arttu.,Kaasinen, Eevi.,Sahu, Biswajyoti.,...&Taipale, Jussi.(2017).Impact of cytosine methylation on DNA binding specificities of human transcription factors.SCIENCE,356(6337). |
MLA | Yin, Yimeng,et al."Impact of cytosine methylation on DNA binding specificities of human transcription factors".SCIENCE 356.6337(2017). |
条目包含的文件 | 条目无相关文件。 |
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