Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aam9321 |
Nucleic acid detection with CRISPR-Cas13a/C2c2 | |
Gootenberg, Jonathan S.1,2,3,4,5; Abudayyeh, Omar O.1,2,3,4,6; Lee, Jeong Wook7; Essletzbichler, Patrick1,2,3,4; Dy, Aaron J.1,4,8; Joung, Julia1,2,3,4; Verdine, Vanessa1,2,3,4; Donghia, Nina7; Daringer, Nichole M.8; Freije, Catherine A.1,9; Myhrvold, Cameron1,9; Bhattacharyya, Roby P.1; Livny, Jonathan1; Regev, Aviv1,10; Koonin, Eugene V.11; Hung, Deborah T.1; Sabeti, Pardis C.1,9,12,13; Collins, James J.1,4,6,7,8; Zhang, Feng1,2,3,4 | |
2017-04-28 | |
发表期刊 | SCIENCE
![]() |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 356期号:6336页码:438-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous ribonuclease activity upon target recognition. We combine the collateral effect of Cas13a with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity. We use this Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumor DNA. Furthermore, SHERLOCK reaction reagents can be lyophilized for cold-chain independence and long-term storage and be readily reconstituted on paper for field applications. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000400143000054 |
WOS关键词 | CIRCULATING TUMOR DNA ; CRISPR-CAS SYSTEMS ; ZIKA VIRUS ; AMPLIFICATION ; PROTEINS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195922 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Broad Inst & MIT Harvard, Cambridge, MA 02142 USA; 2.MIT, McGovern Inst Brain Res, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 3.MIT, Dept Brain & Cognit Sci, E25-618, Cambridge, MA 02139 USA; 4.MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 5.Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA; 6.MIT, Dept Hlth Sci & Technol, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 7.Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA; 8.MIT, Inst Med Engn & Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 9.Harvard Univ, Dept Organism & Evolutionary Biol, Ctr Syst Biol, Cambridge, MA 02138 USA; 10.MIT, Dept Biol, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 11.NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA; 12.Harvard Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA; 13.Howard Hughes Med Inst, Chevy Chase, MD 20815 USA |
推荐引用方式 GB/T 7714 | Gootenberg, Jonathan S.,Abudayyeh, Omar O.,Lee, Jeong Wook,et al. Nucleic acid detection with CRISPR-Cas13a/C2c2[J]. SCIENCE,2017,356(6336):438-+. |
APA | Gootenberg, Jonathan S..,Abudayyeh, Omar O..,Lee, Jeong Wook.,Essletzbichler, Patrick.,Dy, Aaron J..,...&Zhang, Feng.(2017).Nucleic acid detection with CRISPR-Cas13a/C2c2.SCIENCE,356(6336),438-+. |
MLA | Gootenberg, Jonathan S.,et al."Nucleic acid detection with CRISPR-Cas13a/C2c2".SCIENCE 356.6336(2017):438-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论