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DOI | 10.1126/science.aag3218 |
Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state | |
Snijder, Joost1,2,3,7; Schuller, Jan M.4,8; Wiegard, Anika5; Lossl, Philip1,2,3; Schmelling, Nicolas5; Axmann, Ilka M.5; Plitzko, Juergen M.4; Foerster, Friedrich4,6; Heck, Albert J. R.1,2,3 | |
2017-03-17 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6330页码:1181-1184 |
文章类型 | Article |
语种 | 英语 |
国家 | Netherlands; Germany; USA |
英文摘要 | Cyanobacteria have a robust circadian oscillator, known as the Kai system. Reconstituted from the purified protein components KaiC, KaiB, and KaiA, it can tick autonomously in the presence of adenosine 5'-triphosphate (ATP). The KaiC hexamers enter a natural 24-hour reaction cycle of autophosphorylation and assembly with KaiB and KaiA in numerous diverse forms. We describe the preparation of stoichiometrically well-defined assemblies of KaiCB and KaiCBA, as monitored by native mass spectrometry, allowing for a structural characterization by single-particle cryo-electron microscopy and mass spectrometry. Our data reveal details of the interactions between the Kai proteins and provide a structural basis to understand periodic assembly of the protein oscillator. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000396351200037 |
WOS关键词 | CLOCK PROTEIN KAIB ; CRYSTAL-STRUCTURE ; PHOSPHORYLATION ; DYNAMICS ; COMPLEX ; KAIABC ; RHYTHM ; EXPRESSION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195639 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Univ Utrecht, Biomol Mass Spectrometry & Prote, Padualaan 8, NL-3584 CH Utrecht, Netherlands; 2.Univ Utrecht, Netherlands Prote Ctr, Bijvoet Ctr Biomol Res, Padualaan 8, NL-3584 CH Utrecht, Netherlands; 3.Univ Utrecht, Utrecht Inst Pharmaceut Sci, Padualaan 8, NL-3584 CH Utrecht, Netherlands; 4.Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany; 5.Heinrich Heine Univ Dusseldorf, Inst Synthet Microbiol, Cluster Excellence Plant Sci CEPLAS, D-40225 Dusseldorf, Germany; 6.Univ Utrecht, Bijvoet Ctr Biomol Res, Cryoelectron Microscopy, Padualaan 8, NL-3584 CH Utrecht, Netherlands; 7.Univ Washington, Dept Biochem, Seattle, WA 98195 USA; 8.Max Planck Inst Biochem, Dept Struct Cell Biol, D-82152 Martinsried, Germany |
推荐引用方式 GB/T 7714 | Snijder, Joost,Schuller, Jan M.,Wiegard, Anika,et al. Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state[J]. SCIENCE,2017,355(6330):1181-1184. |
APA | Snijder, Joost.,Schuller, Jan M..,Wiegard, Anika.,Lossl, Philip.,Schmelling, Nicolas.,...&Heck, Albert J. R..(2017).Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state.SCIENCE,355(6330),1181-1184. |
MLA | Snijder, Joost,et al."Structures of the cyanobacterial circadian oscillator frozen in a fully assembled state".SCIENCE 355.6330(2017):1181-1184. |
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