Engineering the ribosomal DNA in a megabase synthetic chromosome

doi:10.1126/science.aaf3981

GSTDTAP > 地球科学

DOI	10.1126/science.aaf3981
	Engineering the ribosomal DNA in a megabase synthetic chromosome
	Zhang, Weimin 1,2,3; Zhao, Guanghou 1,2,4; Luo, Zhouqing 1,2; Lin, Yicong 1,2; Wang, Lihui 1,2; Guo, Yakun 1,2; Wang, Ann 1,2,15; Jiang, Shuangying 1,2; Jiang, Qingwen 1,2; Gong, Jianhui 5; Wang, Yun 5; Hou, Sha 1,2; Huang, Jing 1,2; Li, Tianyi 1,2,3; Qin, Yiran 1,2; Dong, Junkai 1,2; Qin, Qin 1,2; Zhang, Jiaying 1,2,3; Zou, Xinzhi 1,2; He, Xi 1,2; Zhao, Li 1,2; Xiao, Yibo 1,2; Xu, Meng 1,2; Cheng, Erchao 1,2; Huang, Ning 1,2; Zhou, Tong 1,2; Shen, Yue 5,6,7; Walker, Roy 6; Luo, Yisha 6; Kuang, Zheng 8,9; Mitchell, Leslie A.8,9; Yang, Kun 10,11; Richardson, Sarah M.10,11; Wu, Yi 12; Li, Bing-Zhi 12; Yuan, Ying-Jin 12; Yang, Huanming 5,13; Lin, Jiwei 14; Chen, Guo-Qiang 1,2; Wu, Qingyu 1,2; Bader, Joel S.10,11; Cai, Yizhi 6; Boeke, Jef D.8,9; Dai, Junbiao 1,2
	2017-03-10
发表期刊	SCIENCE
ISSN	0036-8075
EISSN	1095-9203
出版年	2017
卷号	355 期号:6329
文章类型	Article
语种	英语
国家	Peoples R China; Scotland; USA
英文摘要	We designed and synthesized a 976,067-base pair linear chromosome, synXII, based on native chromosome XII in Saccharomyces cerevisiae. SynXII was assembled using a twostep method, specified by successive megachunk integration and meiotic recombinationmediated assembly, producing a functional chromosome in S. cerevisiae. Minor growth defect " bugs" detected in synXII, caused by deletion of tRNA genes, were rescued by introducing an ectopic copy of a single tRNA gene. The ribosomal gene cluster (rDNA) on synXII was left intact during the assembly process and subsequently replaced by a modified rDNA unit used to regenerate rDNA at three distinct chromosomal locations. The signature sequences within rDNA, which can be used to determine species identity, were swapped to generate a Saccharomyces synXII strain that would be identified as Saccharomyces bayanus by standard DNA barcoding procedures.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000396348900036
WOS关键词	SACCHAROMYCES-CEREVISIAE ; MOLECULAR-GENETICS ; YEAST ; GENES ; NUCLEOLUS ; SEQUENCE ; DESIGN
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/195587
专题	地球科学资源环境科学气候变化
作者单位	1.Tsinghua Univ, Minist Educ, Key Lab Ind Biocatalysis, Beijing 100084, Peoples R China; 2.Tsinghua Univ, Ctr Synthet & Syst Biol, Sch Life Sci, Beijing 100084, Peoples R China; 3.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Beijing 100084, Peoples R China; 4.Peking Univ, Coll Life Sci, Peking Univ Tsinghua Univ Natl Inst Biol Sci Join, Beijing 100871, Peoples R China; 5.BGI Shenzhen, Shenzhen 518083, Peoples R China; 6.Univ Edinburgh, Sch Biol Sci, Edinburgh EH9 3BF, Midlothian, Scotland; 7.BGI Qingdao, Qingdao 266555, Peoples R China; 8.NYU, Langone Med Ctr, Inst Syst Genet, New York, NY 10011 USA; 9.NYU, Langone Med Ctr, Dept Biochem & Mol Pharmacol, New York, NY 10011 USA; 10.Johns Hopkins Univ, High Throughput Biol Ctr, Baltimore, MD 21205 USA; 11.Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD USA; 12.Tianjin Univ, Key Lab Syst Bioengn, Minist Educ,Sch Chem Engn & Technol, SynBio Res Platform,Collaborat Innovat Ctr Chem S, Tianjin 300072, Peoples R China; 13.James D Watson Inst Genome Sci, Hangzhou 310058, Zhejiang, Peoples R China; 14.Wuxi Qinglan Biotechnol Inc, Yixing 214200, Jiangsu, Peoples R China; 15.Icahn Sch Med Mt Sinai, New York, NY 10029 USA
推荐引用方式 GB/T 7714	Zhang, Weimin,Zhao, Guanghou,Luo, Zhouqing,et al. Engineering the ribosomal DNA in a megabase synthetic chromosome[J]. SCIENCE,2017,355(6329).
APA	Zhang, Weimin.,Zhao, Guanghou.,Luo, Zhouqing.,Lin, Yicong.,Wang, Lihui.,...&Dai, Junbiao.(2017).Engineering the ribosomal DNA in a megabase synthetic chromosome.SCIENCE,355(6329).
MLA	Zhang, Weimin,et al."Engineering the ribosomal DNA in a megabase synthetic chromosome".SCIENCE 355.6329(2017).