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DOI | 10.1126/science.aah7124 |
Hidden dynamics in the unfolding of individual bacteriorhodopsin proteins | |
Yu, Hao1,2; Siewny, Matthew G. W.1,2,3; Edwards, Devin T.1,2; Sanders, Aric W.4; Perkins, Thomas T.1,2,5 | |
2017-03-03 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6328页码:945-949 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Protein folding occurs as a set of transitions between structural states within an energy landscape. An oversimplified view of the folding process emerges when transiently populated states are undetected because of limited instrumental resolution. Using force spectroscopy optimized for 1-microsecond resolution, we reexamined the unfolding of individual bacteriorhodopsin molecules in native lipid bilayers. The experimental data reveal the unfolding pathway in unprecedented detail. Numerous newly detected intermediates-many separated by as few as two or three amino acids-exhibited complex dynamics, including frequent refolding and state occupancies of <10 mu s. Equilibrium measurements between such states enabled the folding free-energy landscape to be deduced. These results sharpen the picture of the mechanical unfolding of membrane proteins and, more broadly, enable experimental access to previously obscured protein dynamics. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000395181700038 |
WOS关键词 | MOLECULE FORCE SPECTROSCOPY ; SINGLE CALMODULIN MOLECULES ; FOLDING ENERGY LANDSCAPES ; MEMBRANE-PROTEINS ; MICROSCOPY ; STABILITY ; VELOCITY ; NETWORK ; TITIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195546 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.NIST, JILA, Boulder, CO 80309 USA; 2.Univ Colorado, Boulder, CO 80309 USA; 3.Univ Colorado, Dept Phys, Boulder, CO 80309 USA; 4.Natl Inst Stand & Technol, Radio Frequency Technol Div, Boulder, CO 80305 USA; 5.Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA |
推荐引用方式 GB/T 7714 | Yu, Hao,Siewny, Matthew G. W.,Edwards, Devin T.,et al. Hidden dynamics in the unfolding of individual bacteriorhodopsin proteins[J]. SCIENCE,2017,355(6328):945-949. |
APA | Yu, Hao,Siewny, Matthew G. W.,Edwards, Devin T.,Sanders, Aric W.,&Perkins, Thomas T..(2017).Hidden dynamics in the unfolding of individual bacteriorhodopsin proteins.SCIENCE,355(6328),945-949. |
MLA | Yu, Hao,et al."Hidden dynamics in the unfolding of individual bacteriorhodopsin proteins".SCIENCE 355.6328(2017):945-949. |
条目包含的文件 | 条目无相关文件。 |
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