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DOI | 10.1126/science.aah4712 |
RPA binds histone H3-H4 and functions in DNA replication-coupled nucleosome assembly | |
Liu, Shaofeng1,2,3; Xu, Zhiyun1,2; Leng, He1,2,4; Zheng, Pu1,2; Yang, Jiayi1,2; Chen, Kaifu5; Feng, Jianxun1,2; Li, Qing1,2 | |
2017-01-27 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6323页码:415-419 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China; USA |
英文摘要 | DNA replication-coupled nucleosome assembly is essential to maintain genome integrity and retain epigenetic information. Multiple involved histone chaperones have been identified, but how nucleosome assembly is coupled to DNA replication remains elusive. Here we show that replication protein A (RPA), an essential replisome component that binds single-stranded DNA, has a role in replication-coupled nucleosome assembly. RPA directly binds free H3-H4. Assays using a synthetic sequence that mimics freshly unwound single-stranded DNA at replication fork showed that RPA promotes DNA-(H3-H4) complex formation immediately adjacent to double-stranded DNA. Further, an RPA mutant defective in H3-H4 binding exhibited attenuated nucleosome assembly on nascent chromatin. Thus, we propose that RPA functions as a platform for targeting histone deposition to replication fork, through which RPA couples nucleosome assembly with ongoing DNA replication. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000393172800044 |
WOS关键词 | SINGLE-STRANDED-DNA ; DAMAGE RESPONSE ; CHROMATIN ; YEAST ; ACETYLATION ; INHERITANCE ; INVITRO ; PROTEIN |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195311 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Peking Univ, Sch Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China; 2.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China; 3.Tsinghua Univ, Sch Life Sci, Peking Univ Tsinghua Univ Natl Inst Biol Sci Join, Beijing 100084, Peoples R China; 4.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China; 5.Houston Methodist, DeBakey Heart & Vasc Ctr, Ctr Cardiovasc Regenerat, Houston, TX 77030 USA |
推荐引用方式 GB/T 7714 | Liu, Shaofeng,Xu, Zhiyun,Leng, He,et al. RPA binds histone H3-H4 and functions in DNA replication-coupled nucleosome assembly[J]. SCIENCE,2017,355(6323):415-419. |
APA | Liu, Shaofeng.,Xu, Zhiyun.,Leng, He.,Zheng, Pu.,Yang, Jiayi.,...&Li, Qing.(2017).RPA binds histone H3-H4 and functions in DNA replication-coupled nucleosome assembly.SCIENCE,355(6323),415-419. |
MLA | Liu, Shaofeng,et al."RPA binds histone H3-H4 and functions in DNA replication-coupled nucleosome assembly".SCIENCE 355.6323(2017):415-419. |
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