Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aak9704 |
A global view of meiotic double-strand break end resection | |
Mimitou, Eleni P.1; Yamada, Shintaro1; Keeney, Scott1,2 | |
2017-01-06 | |
发表期刊 | SCIENCE
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ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2017 |
卷号 | 355期号:6320页码:40-+ |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | DNA double-strand breaks that initiate meiotic recombination are exonucleolytically processed. This 5'. 3' resection is a central, conserved feature of recombination but remains poorly understood. To address this lack, we mapped resection endpoints genome-wide at high resolution in Saccharomyces cerevisiae. Full-length resection requires Exo1 exonuclease and the DSB-responsive kinase Tel1, but not Sgs1 helicase. Tel1 also promotes efficient and timely resection initiation. Resection endpoints display pronounced heterogeneity between genomic loci that reflects a tendency for nucleosomes to block Exo1, yet Exo1 also appears to digest chromatin with high processivity and at rates similar to naked DNA in vitro. This paradox points to nucleosome destabilization or eviction as a defining feature of the meiotic resection landscape. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000391739900037 |
WOS关键词 | SACCHAROMYCES-CEREVISIAE ; CELL-CYCLE ; DNA ; EXO1 ; RECOMBINATION ; NUCLEOSOME ; MEIOSIS ; RESOLUTION ; INITIATION ; PATHWAYS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/195153 |
专题 | 地球科学 资源环境科学 气候变化 |
作者单位 | 1.Mem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10065 USA; 2.Howard Hughes Med Inst, Mem Sloan Kettering Canc Ctr, New York, NY 10065 USA |
推荐引用方式 GB/T 7714 | Mimitou, Eleni P.,Yamada, Shintaro,Keeney, Scott. A global view of meiotic double-strand break end resection[J]. SCIENCE,2017,355(6320):40-+. |
APA | Mimitou, Eleni P.,Yamada, Shintaro,&Keeney, Scott.(2017).A global view of meiotic double-strand break end resection.SCIENCE,355(6320),40-+. |
MLA | Mimitou, Eleni P.,et al."A global view of meiotic double-strand break end resection".SCIENCE 355.6320(2017):40-+. |
条目包含的文件 | 条目无相关文件。 |
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