Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1289/EHP4583 |
Influence of Nanomolar Deltamethrin on the Hallmarks of Primary Cultured Cortical Neuronal Network and the Role of Ryanodine Receptors | |
Zheng, Jing1,2; Yu, Yiyi1; Feng, Wei2; Li, Jing1; Liu, Ju1; Zhang, Chunlei1; Dong, Yao2; Pessah, Isaac N.2; Cao, Zhengyu1 | |
2019-06-01 | |
发表期刊 | ENVIRONMENTAL HEALTH PERSPECTIVES |
ISSN | 0091-6765 |
EISSN | 1552-9924 |
出版年 | 2019 |
卷号 | 127期号:6 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China; USA |
英文摘要 | BACKGROUND: The pyrethroid deltamethrin (DM) is broadly used for insect control. Although DM hyperexcites neuronal networks by delaying inactivation of axonal voltage-dependent Na+ channels, this mechanism is unlikely to mediate neurotoxicity at lower exposure levels during critical perinatal periods in mammals. OBJECTIVES: We aimed to identify mechanisms by which acute and subchronic DM altered axonal and dendritic growth, patterns of synchronous Ca2+ oscillations (SCOs), and electrical spike activity (ESA) functions critical to neuronal network formation. METHODS: Measurements of SCOs using Ca2+ imaging, ESA using microelectrode array (MEA) technology, and dendritic complexity using Sholl analysis were performed in primary murine cortical neurons from wild-type (WT) and/or ryanodine receptor 1 (RyR1(T4826I/T4826I)) mice between 5 and 14 d in vitro (DIV). [H-3]ryanodine binding analysis and a single-channel voltage clamp were utilized to measure engagement of RyRs as a direct target of DM. RESULTS: Neuronal networks responded to DM (30-70 nM) as early as 5 DIV, reducing SCO amplitude and depressing ESA and burst frequencies by 60-70%. DM (10-300 nM) enhanced axonal growth in a nonmonotonic manner. DM >= 100 nM enhanced dendritic complexity. DM stabilized channel open states of RyR1, RyR2, and cortical preparations expressing all three isoforms. DM (30 nM) altered gating kinetics of RyR1 channels, increasing mean open time, decreasing mean closed time, and thereby enhancing overall open probability. SCO patterns from cortical networks expressing RyR1(T4826I/T4826I) were more responsive to DM than WT. RyR1(T4826I/T4826I) neurons showed inherently longer axonal lengths than WT neurons and maintained less length-promoting responses to nanomolar DM. CONCLUSIONS: Our findings suggested that RyRs were sensitive molecular targets of DM with functional consequences likely relevant for mediating abnormal neuronal network connectivity in vitro. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000474528700007 |
WOS关键词 | II PYRETHROID INSECTICIDE ; HUMAN BREAST-MILK ; HIPPOCAMPAL-NEURONS ; CALCIUM OSCILLATIONS ; PESTICIDE EXPOSURE ; CA2+ OSCILLATIONS ; AGRICULTURAL AREA ; AXONAL GROWTH ; CHANNEL ; MECHANISMS |
WOS类目 | Environmental Sciences ; Public, Environmental & Occupational Health ; Toxicology |
WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health ; Toxicology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/183724 |
专题 | 资源环境科学 |
作者单位 | 1.China Pharmaceut Univ, Sch Tradit Pharm, Dept TCM Pharmacol, Stale Key Lab Nat Med,Jiangsu Prov Key Lab TCM Ev, Nanjing, Jiangsu, Peoples R China; 2.Univ Calif Davis, Sch Vet Med, Dept Mol Biosci, 1020 VM3B 1089 Vet Med Way, Davis, CA 95616 USA |
推荐引用方式 GB/T 7714 | Zheng, Jing,Yu, Yiyi,Feng, Wei,et al. Influence of Nanomolar Deltamethrin on the Hallmarks of Primary Cultured Cortical Neuronal Network and the Role of Ryanodine Receptors[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2019,127(6). |
APA | Zheng, Jing.,Yu, Yiyi.,Feng, Wei.,Li, Jing.,Liu, Ju.,...&Cao, Zhengyu.(2019).Influence of Nanomolar Deltamethrin on the Hallmarks of Primary Cultured Cortical Neuronal Network and the Role of Ryanodine Receptors.ENVIRONMENTAL HEALTH PERSPECTIVES,127(6). |
MLA | Zheng, Jing,et al."Influence of Nanomolar Deltamethrin on the Hallmarks of Primary Cultured Cortical Neuronal Network and the Role of Ryanodine Receptors".ENVIRONMENTAL HEALTH PERSPECTIVES 127.6(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论